DAO 84:209-217 (2009)  -  DOI: https://doi.org/10.3354/dao02045

Identification of Vibrio harveyi isolated from diseased cultured wedge sole Dicologoglossa cuneata

Jose R. López1,*, Elena de la Roca1, Soledad Núñez2, Roberto de la Herran3, José I. Navas1, Manuel Manchado4, Marcelino Herrera1, Alicia E. Toranzo2

1IFAPA Centro Agua del Pino, Junta de Andalucía, 21450 Huelva, Spain
2Departamento de Microbiología y Parasitología, Facultad de Biología e Instituto de Acuicultura, Universidad de Santiago, 15782 Santiago de Compostela, Spain
3Departamento de Genética, Facultad de Ciencias, Universidad de Granada, 18071 Granada, Spain
4IFAPA Centro El Toruño, Junta de Andalucía, 11500 Cádiz, Spain

ABSTRACT: We report the first isolation of Vibrio harveyi from wedge sole Dicologoglossa cuneata. The pathogen was recovered from ulcers and internal organs of ailing cultured fish, from 7 different outbreaks between 2004 and 2006. The 15 isolates found were phenotypically characterized using biochemical tests and BIOLOG GN plates, which revealed high phenotypic diversity. Diagnosis was confirmed with PCR using V. harveyi specific primers and partial 16S and 23S rRNA gene sequencing. A virulence evaluation of the isolates was also performed using fry and juvenile wedge sole. Significant mortalities were recorded by intraperitoneal injection; however, no mortalities were recorded by bath immersion.


KEY WORDS: Vibrio harveyi · Characterization · Wedge sole · Dicologoglossa cuneata · Virulence


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Cite this article as: López JR, de la Roca E, Núñez S, de la Herran R and others (2009) Identification of Vibrio harveyi isolated from diseased cultured wedge sole Dicologoglossa cuneata. Dis Aquat Org 84:209-217. https://doi.org/10.3354/dao02045

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