DAO 85:167-173 (2009)  -  DOI: https://doi.org/10.3354/dao02075

Parvo-like virus in the hepatopancreas of freshwater prawns Macrobrachium rosenbergii cultivated in Thailand

Warachin Gangnonngiw1,2, Wansika Kiatpathomchai1,2, Siriporn Sriurairatana1, Kesinee Laisutisan3, Niti Chuchird3, Chalor Limsuwan3, Timothy W. Flegel1,4,*

1Centex Shrimp, Chalerm Prakiat Building, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand
2National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Klong 1, Klong Luang, Pratum Thani 12120, Thailand
3Department of Fishery Biology, Faculty of Fisheries, Kasetsart University, Pahonyothin Road, Bangkok 10900, Thailand
4Department of Biotechnology, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand
*Corresponding author. Email:

ABSTRACT: A survey of cultivated giant freshwater prawns Macrobrachium rosenbergii from Thailand revealed the presence of unusual spherical to ovoid inclusions in nuclei of hepatopancreas tubule epithelial cells. These began as small eosinophilic inclusions that became more basophilic as they increased in size. They were present in both R-cells and E-cells but were largest and deeply basophilic only in the E-cells. Confocal laser microscopy revealed that stained nucleic acid fluorescence from the inclusions was lost by treatment with DNase I specific for double- and single-stranded DNA and also lost or reduced by treatment with mungbean nuclease specific for single-stranded nucleic acids. Transmission electron microscopy (TEM) revealed that the inclusions contained tightly packed, unenveloped, viral-like particles of approximately 25 to 30 nm diameter, resembling those produced by shrimp parvoviruses. However, PCR, in situ hybridization and immunohistochemical tests for shrimp parvoviruses previously reported from Thailand were all negative. These results suggested that the inclusions contained a parvo-like virus, not previously reported from M. rosenbergii in Thailand.


KEY WORDS: Macrobrachium rosenbergii · Hepatopancreas · Parvo-like virus · Histopathology · Electron microscopy


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Cite this article as: Gangnonngiw W, Kiatpathomchai W, Sriurairatana S, Laisutisan K, Chuchird N, Limsuwan C, Flegel TW (2009) Parvo-like virus in the hepatopancreas of freshwater prawns Macrobrachium rosenbergii cultivated in Thailand. Dis Aquat Org 85:167-173. https://doi.org/10.3354/dao02075

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