DAO 86:213-221 (2009)  -  DOI: https://doi.org/10.3354/dao02108

Genetic and serological typing of European infectious haematopoietic necrosis virus (IHNV) isolates

Tove Johansson1,6,*, Katja Einer-Jensen1, William Batts2, Peter Ahrens3, Carina Björkblom4, Gael Kurath2, Harry Björklund5, Niels Lorenzen1

1National Veterinary Institute, Technical University of Denmark, Hangøvej 2, 8200 Århus N, Denmark
2Western Fisheries Research Center, US Geological Survey, 6505 NE 65th Street, Seattle, Washington 98115, USA
3Statens Serum Institut, Artillerivej 5, 2300 Copenhagen, Denmark
4Laboratory of Aquatic Pathobiology, Åbo Akademi University, Artillerigatan 6 A 2, 20520 Åbo, Finland
5Orion Pharma, PO Box 425, 20101 Åbo, Finland
6Present address: Laboratory of Aquatic Pathobiology, Department of Biology, Åbo Akademi University, Artillerigatan 6 A 2, 20520 Åbo, Finland

ABSTRACT: Infectious haematopoietic necrosis virus (IHNV) causes the lethal disease infectious haematopoietic necrosis (IHN) in juvenile salmon and trout. The nucleocapsid (N) protein gene and partial glycoprotein (G) gene (nucleotides 457 to 1061) of the European isolates IT-217A, FR-32/87, DE-DF 13/98 11621, DE-DF 4/99-8/99, AU-9695338 and RU-FR1 were sequenced and compared with IHNV isolates from the North American genogroups U, M and L. In phylogenetic studies the N gene of the Italian, French, German and Austrian isolates clustered in the M genogroup, though in a different subgroup than the isolates from the USA. Analyses of the partial G gene of these European isolates clustered them in the M genogroup close to the root while the Russian isolate clustered in the U genogroup. The European isolates together with US-WRAC and US-Col-80 were also tested in an enzyme-linked immunosorbent assay (ELISA) using monoclonal antibodies (MAbs) against the N protein. MAbs 136-1 and 136-3 reacted equally at all concentrations with the isolates tested, indicating that these antibodies identify a common epitope. MAb 34D3 separated the M and L genogroup isolates from the U genogroup isolate. MAb 1DW14D divided the European isolates into 2 groups. MAb 1DW14D reacted more strongly with DE-DF 13/98 11621 and RU-FR1 than with IT-217A, FR-32/87, DE-DF 4/99-8/99 and AU-9695338. In the phylogenetic studies, the Italian, French, German and Austrian isolates clustered in the M genogroup, whereas in the serological studies using MAbs, the European M genogroup isolates could not be placed in the same specific group. These results indicate that genotypic and serotypic classification do not correlate.


KEY WORDS: Infectious haematopoietic necrosis virus · IHNV · Europe · Nucleocapsid protein · Glycoprotein · Phylogeny · Enzyme-linked immunosorbent assay · ELISA


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Cite this article as: Johansson T, Einer-Jensen K, Batts W, Ahrens P and others (2009) Genetic and serological typing of European infectious haematopoietic necrosis virus (IHNV) isolates. Dis Aquat Org 86:213-221. https://doi.org/10.3354/dao02108

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