DAO 89:17-27 (2010)  -  DOI: https://doi.org/10.3354/dao02183

Development of a monoclonal antibody against viral haemorrhagic septicaemia virus (VHSV) genotype IVa

T. Ito1,*, N. J. Olesen2, H. F. Skall2, M. Sano3, J. Kurita1, K. Nakajima4, T. Iida3

1Tamaki Station, Aquatic Animal Health Division, National Research Institute of Aquaculture, Fisheries Research Agency, Tamaki, Mie 519-0423, Japan
2Section for Fish Diseases, Division of Poultry, Fish and Fur Animals, National Veterinary Institute, Technical University of Denmark, Hangøvej 2, 8200 Århus N, Denmark
3Aquatic Animal Health Division, National Research Institute of Aquaculture, Fisheries Research Agency, Minami-Ise, Mie 516-0193, Japan
4Aquatic Genomics Research Center, National Research Institute of Fisheries Science, Fisheries Research Agency, Fukuura, Kanazawa, Yokohama, Kanagawa 236-8648, Japan

ABSTRACT: The viral haemorrhagic septicaemia virus (VHSV) comprises 4 major genotypes and a number of subtypes with, in most cases, distinct geographical distribution. A quick and simple detection method that can discriminate the different genotypes is desirable for a quick and more efficient prevention of the spread of genotypes to new geographical areas. A monoclonal antibody (MAb) against VHSV genotype IVa was produced, with the aim of providing a simple method of discriminating this genotype from the other VHSV genotypes (I, II, III and IVb). Balb/c mice were injected with purified VHSV-JF00Ehil (genotype IVa) from diseased farmed Japanese flounder. Ten hybridoma clones secreting monoclonal antibodies (MAbs) against VHSV were established. One of these, MAb VHS-10, reacted only with genotype IVa in indirect fluorescent antibody technique (IFAT) and ELISA. Using cell cultures that were transfected with each of the viral protein genes, it was shown that the MAb VHS-10 recognizes a nonlinear genotype IVa-specific epitope on the VHSV N-protein.


KEY WORDS: VHSV · Genotyping · Monoclonal antibody · Indirect fluorescent antibody technique · IFAT · ELISA


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Cite this article as: Ito T, Olesen NJ, Skall HF, Sano M, Kurita J, Nakajima K, Iida T (2010) Development of a monoclonal antibody against viral haemorrhagic septicaemia virus (VHSV) genotype IVa. Dis Aquat Org 89:17-27. https://doi.org/10.3354/dao02183

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