DAO 89:269-273 (2010)  -  DOI: https://doi.org/10.3354/dao02199

NOTE
Perkinsus marinus in Crassostrea gigas in the Gulf of California

T. L. Enríquez-Espinoza, J. M. Grijalva-Chon*, R. Castro-Longoria, J. Ramos-Paredes

Departamento de Investigaciones Científicas y Tecnológicas, Universidad de Sonora, Colosio entre Reforma y Sahuaripa s/n, Hermosillo, Sonora, Mexico
*Corresponding author. Email:

ABSTRACT: To determine the agent responsible for the massive mortalities of the Pacific oyster Crassostrea gigas in northwest Mexico, 30 oysters were sampled after a severe mortality event in 2006 along the Sonoran coast. Histological analyses revealed the presence of a protozoan and Ray’s fluid thioglycollate medium (RFTM) assays showed the presence of Perkinsus sp., identified as P. marinus from the DNA sequence of the internal transcribed spacer (ITS) of the ribosomal RNA (rRNA) gene complex. PCR analyses for Marteilia refringens, M. sydneyi, and Haplosporidium costale were negative. P. marinus presence in the Pacific oyster may be responsible for massive mortalities of the oyster, along with other environmental factors in the Gulf of California.


KEY WORDS: Perkinsus marinus · Pacific oyster · Crassostrea gigas · Aquaculture · Gulf of California · Ray’s fluid thioglycollate medium · Ribosomal DNA


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Cite this article as: Enríquez-Espinoza TL, Grijalva-Chon JM, Castro-Longoria R, Ramos-Paredes J (2010) Perkinsus marinus in Crassostrea gigas in the Gulf of California. Dis Aquat Org 89:269-273. https://doi.org/10.3354/dao02199

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