DAO 90:63-68 (2010) - DOI: https://doi.org/10.3354/dao02206
Immune response in Lutjanus erythropterus induced by the major outer membrane protein (OmpU) of Vibrio alginolyticus
Shuang-Hu Cai1,2, Shao-Yun Yao1,2, Yi-Shan Lu1,2, Zao-He Wu1,2, Ji-Chang Jian1,2,*, Bei Wang1,2
ABSTRACT: The outer membrane proteins (OMPs) of the marine aquatic animal pathogen Vibrio alginolyticus play an important role in the virulence of the bacterium and are potential candidates for vaccine development. In this study, the major 35.6 kDa OMP of V. alginolyticus was isolated by gel excision from the crude OMP fraction from V. alginolyticus. The sequence of the first 27 amino acid residues from the N-terminal end of the protein is ATV YKD GGT ELL VGG RVE FRG DFI GSD, which has high homology with OmpU proteins from other Vibrio spp. (92%). Lutjanus erythropterus were vaccinated with OmpU, and immunogenicity was confirmed by subsequent western blotting. Enzyme-linked immunosorbent assay (ELISA) analysis demonstrated that OmpU produced an observable antibody response in all sera of the vaccinated fish. L. erythropterus vaccinated with OmpU produced specific antibodies, and were highly resistant to infection with virulent V. alginolyticus. These results indicate that OmpU is an effective vaccine candidate against V. alginolyticus for L. erythropterus.
KEY WORDS: Vibrio alginolyticus · Outer membrane protein · OmpU · Immunogenicity · Vaccine
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Full text in pdf format Erratum | Cite this article as: Cai SH, Yao SY, Lu YS, Wu ZH, Jian JC, Wang B
(2010) Immune response in Lutjanus erythropterus induced by the major outer membrane protein (OmpU) of Vibrio alginolyticus. Dis Aquat Org 90:63-68. https://doi.org/10.3354/dao02206
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