DAO 90:77-83 (2010)  -  DOI: https://doi.org/10.3354/dao02220

NOTE
Successful propagation of shrimp yellow head virus in immortal mosquito cells

Warachin Gangnonngiw1,2, Nipaporn Kanthong3, Timothy W. Flegel1,2,4,*

1Centex Shrimp, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand
2National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Klong 1, Klong Luang, Pratum Thani 12120, Thailand
3Dept. Biotechnology, Faculty of Science and Technology, Rajamangala University of Technology Tawan-ok, Sriracha, Chonburi 20110, Thailand
4Dept. Biotechnology, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand
*Corresponding author. Email:

ABSTRACT: Research on crustacean viruses is hampered by the lack of continuous cell lines susceptible to them. To overcome this problem, we previously challenged immortal mosquito and lepidopteran cell lines with shrimp yellow head virus (YHV), followed by serial, split-passage of whole cells, and showed that this produced cells that persistently expressed YHV antigens. To determine whether such insect cultures positive for YHV antigens could be used to infect shrimp Penaeus monodon with YHV, culture supernatants and whole-cell homogenates were used to challenge shrimp by injection. Shrimp injected with culture supernatants could not be infected. However, shrimp injection-challenged with whole-cell homogenates from Passage 5 (early-passage) of such cultures died with histological and clinical signs typical for yellow head disease (YHD), while homogenates of mock-passaged, YHV-challenged cells did not. By contrast, shrimp challenged with cell homogenates of late-passage cultures became infected with YHV, but survived, suggesting that YHV attenuation had occurred during its long-term serial passage in insect cells. Thus, YHV could be propagated successfully in C6/36 mosquito cells and used at low passage numbers as a source of inoculum to initiate lethal infections in shrimp. This partially solves the problem of lack of continuous shrimp cell lines for cultivation of YHV.


KEY WORDS: Shrimp · Virus · Insect cell line · YHV · Propagation


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Cite this article as: Gangnonngiw W, Kanthong N, Flegel TW (2010) Successful propagation of shrimp yellow head virus in immortal mosquito cells. Dis Aquat Org 90:77-83. https://doi.org/10.3354/dao02220

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