DAO 91:213-221 (2010)  -  DOI: https://doi.org/10.3354/dao02253

Occurrence and seasonality of Vibrio aestuarianus in sediment and Crassostrea gigas haemolymph at two oyster farms in France

Afi Azandégbé1, Matthieu Garnier1, Françoise Andrieux-Loyer2, Roger Kérouel2, Xavier Philippon2, Jean-Louis Nicolas1,*

1Laboratoire de physiologie des Invertébrés, PFOM, and 2Laboratoire Dyneco, IFREMER, Centre de Brest BP70, 29280 Plouzané, France
*Corresponding author. Email:

ABSTRACT:  Vibrio aestuarianus is frequently found in coastal areas and can infect and induce mortalities in the pacific oyster Crassostrea gigas. However, nothing is known about its distribution and seasonality in the estuarine environment, especially where oyster farming is practiced. Its occurrence was investigated in sediment and oyster haemolymph at 2 oyster farms in Brittany (France) over 2 yr during 2 periods, from June to September 2007 and from February to June 2008. Total heterotrophic bacteria (HB) were cultured on marine agar while total Vibrio spp. and V. aestuarianus were selectively numerated using thiosulfate citrate bile salts sucrose agar (TCBS agar) and the species-specific hybridisation method, respectively. PCR was performed to detect V. aestuarianus in sediment when it became unculturable. Both total Vibrio spp. and V. aestuarianus had a seasonal trend. The highest concentrations were recovered in the warmest months. Its abundance ranged from 102 to 4 × 105 CFU ml–1 in haemolymph and from 103 to 1 × 104 CFU g–1 in the sediment. Temperature was the main factor influencing the concentration of Vibrio spp. and V. aestuarianus in the sediment. Thus V. aestuarianus might subsist during the cold seasons in the sediment, from which it can emerge when environmental conditions became favourable.


KEY WORDS: Vibrio aestuarianus · Crassostrea gigas · Sediment · Seasonal abundance · Colony-blot


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Cite this article as: Azandégbé A, Garnier M, Andrieux-Loyer F, Kérouel R, Philippon X, Nicolas JL (2010) Occurrence and seasonality of Vibrio aestuarianus in sediment and Crassostrea gigas haemolymph at two oyster farms in France. Dis Aquat Org 91:213-221. https://doi.org/10.3354/dao02253

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