DAO 93:141-147 (2011)  -  DOI: https://doi.org/10.3354/dao02279

First evidence of Panulirus argus Virus 1 (PaV1) in spiny lobster from Cuba and clinical estimation of its prevalence

Yanis Cruz Quintana1,2,*, Rossanna Rodríguez Canul2, Víctor M. Vidal Martínez2,3

1Laboratorio de Sanidad Acuícola, Centro de Investigaciones Pesqueras, 5ta ave y 246, Barlovento, Playa, Ciudad de La Habana, Cuba
2Laboratorios de Parasitología e Inmunología y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Unidad Mérida, Km 6 Carretera Antigua a Progreso, Cordemex, Mérida, Yucatán 97310, Mexico
3Department of Ecology, Evolution and Marine Biology and Marine Science Institute, University of California, Santa Barbara, California 93106, USA

ABSTRACT: The present study documents the first finding of Panulirus argus Virus 1 (PaV1) in spiny lobster Panulirus argus from Cuba. Samples originated from 2 nursery sites, Matías Keys and Bocas de Alonso Keys, and 2 fishing sites, La Grifa and El Ramajo. Lobsters from the nursery sites (artificial reefs) were collected by SCUBA diving, while those from the fishing sites were collected from artificial shelters known as ‘casitas cubanas’. In these shelters it was observed that healthy lobsters tended to avoid infected lobsters. Prevalence of PaV1 in the sampling sites was assessed by using clinical signs such as lethargy, an opaque reddish shell coloration, and milky white hemolymph with loss of clotting activity. The presence of PaV1 was subsequently confirmed by histology and PCR of tissues and hemolymph samples from suspected individuals. Histological sections of the hepatopancreas, gills, gonads, and gut showed infected hemocytes with hypertrophied nuclei and eosinophilic intranuclear Cowdry type A inclusions. A 499 bp band was observed by PCR. The sequence of the amplified fragments was 96% similar to the PaV1 sequence in GenBank. The overall mean prevalence of PaV1 was 4.48% (range: 0 to 9.3%) after pooling the results of the 4 sampling sites.


KEY WORDS: Crustacea · Spiny lobster · Virus · Panulirus argus Virus 1 · PaV1 · Cuba · Prevalence


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Cite this article as: Cruz Quintana Y, Rodríguez Canul R, Vidal Martínez VM (2011) First evidence of Panulirus argus Virus 1 (PaV1) in spiny lobster from Cuba and clinical estimation of its prevalence. Dis Aquat Org 93:141-147. https://doi.org/10.3354/dao02279

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