DAO 93:149-162 (2011)  -  DOI: https://doi.org/10.3354/dao02288

Mineral phase in shell repair of Manila clam Venerupis philippinarum affected by brown ring disease

Nolwenn Trinkler1, Jean-François Bardeau2, Frédéric Marin3,4, Maylis Labonne5,*, Aurélie Jolivet6, Philippe Crassous6, Christine Paillard1

1IUEM, LEMAR, UMR CNRS/UBO/IRD 6539, Technopole Brest Iroise, 29280 Plouzané, France
2LPEC, UMR CNRS 6087, Université du Maine, 72085 Le Mans Cedex 9, France
3UMR CNRS 5561 Biogéosciences, Dijon, France
4Université de Bourgogne, 6 Boulevard Gabriel, 21000 Dijon, France
5IRD, LEMAR, UMR CNRS/UBO/IRD 6539, Centre IRD Bretagne, BP 70, 29280 Plouzané, France
6Ifremer, STH, Z.I. Pointe du diable, BP 70, 29280 Plouzané, France
*Corresponding author. Email:

ABSTRACT: The mineral phase of shell repair in the Manila clam Venerupis philippinarum affected by brown ring disease (BRD) was characterised at various scales and at various stages of shell repair by confocal Raman microspectrometry and scanning electron microscopy. Spherulitic and quadrangular aragonite microstructures associated with polyene pigments were clearly observed. Von Kossa staining showed that at the beginning of shell repair, hemocytes are filled with insoluble calcium carbonate salts in all fluids and then are transported toward the extrapallial fluids and the repair sites. Our analyses suggest that after a Vibrio tapetis attack and BRD deposit some clams rapidly cover the deposit, resulting in a modification in the microstructure, which could be produced by the participation of both the mantle and hemocytes.


KEY WORDS: Aragonite · Hemocytes · Shell microstructure · Calcium carbonate · Biomineralization · Mollusc · Raman microspectrometry · Scanning electron microscopy


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Cite this article as: Trinkler N, Bardeau JF, Marin F, Labonne M, Jolivet A, Crassous P, Paillard C (2011) Mineral phase in shell repair of Manila clam Venerupis philippinarum affected by brown ring disease. Dis Aquat Org 93:149-162. https://doi.org/10.3354/dao02288

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