DAO 94:73-75 (2011)  -  DOI: https://doi.org/10.3354/dao02312

NOTE
Specific primers for the detection of the black-yeast fungus associated with lethargic crab disease (LCD)

Marcio R. Pie1,2, Walter A. Boeger1,2,*, Luciana Patella1,2, Vânia A. Vicente2,3, Raphael O. Ribeiro1,2, Antonio Ostrensky2,4 

1Departamento de Zoologia, 2Grupo Integrado de Aqüicultura e Estudos Ambientais, 3Departamento de Patologia Básica, and 4Departamento de Zootecnia, Universidade Federal do Paraná, Curitiba, Paraná, Brazil
*Corresponding author. Email:

ABSTRACT: Lethargic crab disease (LCD) is an emerging infirmity that has been causing extensive mortalities in populations of the mangrove land crab Ucides cordatus (Ocypodidae) along the Atlantic coast of Brazil. Previous studies have indicated that LCD is associated with a dematiaceous fungus, Exophiala cancerae de Hoog et al. In the present study, we sequenced the internal transcribed spacer (ITS) of the rDNA region of this black yeast species and developed species-specific PCR primers. Sensitivity tests indicated that the developed protocol is capable of detecting very small amounts of target DNA. Also, the application of the protocol to a variety of other dematiaceous fungi did not generate any false positives. The specific primers provided in the present study represent an important tool for rapidly surveying a large number of crab individuals, as well as environmental samples. Such knowledge will be instrumental in understanding the epidemiological dynamics of LCD.


KEY WORDS: Lethargic crab disease · Diagnosis · Exophiala cancerae


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Cite this article as: Pie MR, Boeger WA, Patella L, Vicente VA, Ribeiro RO, Ostrensky A (2011) Specific primers for the detection of the black-yeast fungus associated with lethargic crab disease (LCD). Dis Aquat Org 94:73-75. https://doi.org/10.3354/dao02312

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