DAO 95:225-231 (2011)  -  DOI: https://doi.org/10.3354/dao02361

Neoparamoeba branchiphila infections in moribund sea urchins Diadema aff. antillarum in Tenerife, Canary Islands, Spain

Iva Dyková1,2,*, Jacob Lorenzo-Morales3,4, Martin Kostka1,2, Basilio Valladares3, Hana Pecková1

1Institute of Parasitology, Biology Centre, Academy of Sciences of the Czech Republic, Branišovská 31, 370 05 České Budějovice, Czech Republic
2Faculty of Science, University of South Bohemia, 370 05 České Budějovice, Czech Republic
3University Institute of Tropical Diseases and Public Health of the Canary Islands, 38207 University of La Laguna, Tenerife, Spain
4Centre for Integrative Physiology, School of Biomedical Sciences, University of Edinburgh, Hugh Robson Building, George Square, Edinburgh EH8 9XD, UK

ABSTRACT: A total of 109 sea urchins from 3 species collected in 2 localities off the coast of Tenerife Island, Spain, were examined for the presence of free-living amoebae in their coelomic fluid. Amoeba trophozoites were isolated exclusively from moribund individuals of long-spined sea urchins Diadema aff. antillarum (Philippi) (Echinoidea, Echinodermata) that manifested lesions related to sea urchin bald disease on their tests (16 out of 56 examined). No amoebae were detected in Arbacia lixula (L.) and Paracentrotus lividus (Lamarck). From the former sea urchin species, 8 strains, established from 10 primary isolates, were identified as Neoparamoeba branchiphila Dyková et al., 2005 using morphological and molecular methods. Results of this study (limited to the screening for free-living amoebae) together with data on agents of sea urchin mortalities reported to date justify the hypothesis that free-living amoebae play an opportunistic role in D. aff. antillarum mortality. The enlargement of the dataset of SSU rDNA sequences brought new insight into the phylogeny of ­Neoparamoeba species.


KEY WORDS: Neoparamoeba · Diadema aff. antillarum · SSU rDNA phylogeny · Tenerife


Full text in pdf format 
Cite this article as: Dyková I, Lorenzo-Morales J, Kostka M, Valladares B, Pecková H (2011) Neoparamoeba branchiphila infections in moribund sea urchins Diadema aff. antillarum in Tenerife, Canary Islands, Spain. Dis Aquat Org 95:225-231. https://doi.org/10.3354/dao02361

Export citation
Mail this link - Contents Mailing Lists - RSS
- -