DAO 96:55-60 (2011)  -  DOI: https://doi.org/10.3354/dao02353

Infection prevalence and phylogenetic analysis of Perkinsus olseni in Ruditapes philippinarum from East China

Shao-qiang Wu, Cai-xia Wang, Xiang-mei Lin*, Zong-xiang Wang, Xiao-fei Li, Jian Liu, Jun-hua Deng, Song-yin Qiu

Institute of Animal and Plant Quarantine, Chinese Academy of Inspection and Quarantine, Beijing 100029, PR China

ABSTRACT: The prevalence of Perkinsus sp. infection in Manila clam Ruditapes philippinarum was investigated in the coastal areas of east China. Thirteen groups of clams were collected from 5 sites: Dandong and Qingdao Bays (Yellow Sea), Weifang Bay (Bohai Sea), and Ningbo and Fuzhou Bays (East China Sea). The clams were tested for perkinsosis infection using Ray’s fluid thioglycollate medium culture assay. Perkinsus sp. was found in samples from all 5 sites from May 2008 to May 2009. Infection prevalence ranged from 43.75 to 95.83%, and was significantly higher in October than in May. The only 3 uninfected groups of clams were collected from Weifang Bay, the site farthest from the ocean. There was no difference in the prevalence of infection among the remaining 4 sites. The conserved internal transcribed spacer regions of the ribosomal RNA gene complex in each of the Perkinsus sp. isolates were amplified by PCR. The resulting amplicons were sequenced and phylogenetically analyzed. All the Perkinsus isolates were identified as Perkinsus olseni.


KEY WORDS: Perkinsus olseni · Ruditapes philippinarum · Infection prevalence · Phylogenetic analysis · China


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Cite this article as: Wu S, Wang C, Lin X, Wang Z and others (2011) Infection prevalence and phylogenetic analysis of Perkinsus olseni in Ruditapes philippinarum from East China. Dis Aquat Org 96:55-60. https://doi.org/10.3354/dao02353

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