DAO 96:105-112 (2011)  -  DOI: https://doi.org/10.3354/dao02388

Analysis of variable genomic loci in white spot ­syndrome virus to predict its origins in Procambarus clarkii crayfish farmed in China

Wei Zeng2, Yong Zeng1,*, Rong-Mei Fei3, Ling-Bing Zeng4, Kai-Jin Wei4

1College of Chemistry and Biology, Yantai University, Shandong 264005, PR China
2No. 2 High School of Yantai, Shandong 264000, PR China
3College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China
4Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Jingzhou, Hubei 434000, PR China

ABSTRACT: Variable genomic loci were examined in 4 white spot syndrome virus (WSSV) isolates (08HB, 09HB, 08JS and 09JS) from Procambarus clarkii crayfish collected from Jiangsu and Hubei Provinces in China in 2008 and 2009. In ORF75, sequence variation detected in the 4 isolates, as well as in isolates sequenced previously, suggested that WSSV might have segregated into 2 lineages since first emerging as a serious pathogen of farmed shrimp in East Asia in the early-mid 1990s, with one lineage remaining in East Asia and the other separating to South Asia. In ORF23/24, deletions of 9.31, 10.97, or 11.09 kb were evident compared to a reference isolate from Taiwan (WSSV-TW), and, in ORF14/15, deletions of 5.14 or 5.95 kb were evident compared to a reference isolate from Thailand with the largest genome size (TH-96-II). With respect to these genome characteristics, the crayfish isolates 08HB, 09HB and 08JS were similar to WSSV-TW and the isolate 09JS was similar to a reference isolate from China (WSSV-CN). In addition to these loci, sequence variation was evident in ORF94 and ORF125 that might be useful for differentiating isolates and in epidemiological tracing of WSSV spread in crayfish farmed in China. However, as all 4 crayfish isolates possessed a Homologous Region 9 sequence identical to isolate WSSV-TW and another Thailand isolate (WSSV-TH), and as their transposase sequence was identical to isolates WSSV-CN and WSSV-TH, these 2 loci were not useful in predicting their origins.


KEY WORDS: White spot syndrome virus · Crayfish · Variable genomic loci · Spread


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Cite this article as: Zeng W, Zeng Y, Fei RM, Zeng LB, Wei KJ (2011) Analysis of variable genomic loci in white spot ­syndrome virus to predict its origins in Procambarus clarkii crayfish farmed in China. Dis Aquat Org 96:105-112. https://doi.org/10.3354/dao02388

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