DAO 96:157-167 (2011)  -  DOI: https://doi.org/10.3354/dao02384

Disseminated neoplasia in cockles Cerastoderma edule: ultrastructural characterisation and effects on haemolymph cell parameters

Seila Díaz1, Tristan Renault2, Antonio Villalba1, María Jesús Carballal1,*

1Centro de Investigacións Mariñas, Consellería do Mar, Xunta de Galicia, 36620 Vilanova de Arousa, Spain
2Institut Français de Recherche pour l’Exploitation de la Mer (IFREMER), Laboratoire de Génétique et Pathologie, Ronce les Bains, 17390 La Tremblade, France

ABSTRACT: Disseminated neoplasia (DN) has been detected in cockles from various beds in Galicia (NW Spain). A study was performed to characterise cockle neoplastic cell ultrastructure and to evaluate the effect of this disease at different severity stages on various haemolymph cell parameters. Examination of cockle neoplastic cells with transmission electron microscopy (TEM) showed round shapes and a lack of pseudopods, a high nucleus:cytoplasm diameter ratio, Golgi complexes, abundant mitochondria, ribosomes, and numerous endoplasmic reticulum tubes and electron-lucent vesicles. Various haemolymph cell parameters (cell mortality, non-specific esterase and lysosome biovolume, reactive oxygen intermediates [ROI] production, phagocytosis ability, intracellular Ca2+ and actin levels) were compared between DN severity categories by flow cytometry; haemocyte mortality, non-specific esterase activities and lysosome biovolume were found to be higher with increasing DN severity. The phagocytic ability of neoplastic cells was sharply reduced with regard to haemocytes. The cytoplasmic-free Ca2+ level was higher and actin content lower in haemolymph cells of diseased cockles compared to unaffected ones. A significant increase in ROI production was detected in later stages of disease progression.


KEY WORDS: Disseminated neoplasia · Cerastoderma edule · Non-specific esterases · Lysosome · Respiratory burst · Phagocytosis · Cytoplasmic free Ca2+ · Actin


Full text in pdf format 
Cite this article as: Díaz S, Renault T, Villalba A, Carballal MJ (2011) Disseminated neoplasia in cockles Cerastoderma edule: ultrastructural characterisation and effects on haemolymph cell parameters. Dis Aquat Org 96:157-167. https://doi.org/10.3354/dao02384

Export citation
Mail this link - Contents Mailing Lists - RSS
- -