DAO 96:195-207 (2011)  -  doi:10.3354/dao02370

Major capsid protein gene sequence analysis of the Santee-Cooper ranaviruses DFV, GV6, and LMBV

S. Ohlemeyer1,*, R. Holopainen2, H. Tapiovaara2, S. M. Bergmann1, H. Schütze1

1Institute of Infectology, Federal Research Institute for Animal Health, Friedrich-Loeffler-Institut, Südufer 10, 17493 Greifswald-Insel Riems, Germany
2Finnish Food Safety Authority Evira, Mustialankatu 3, 00790 Helsinki, Finland

ABSTRACT: The Santee-Cooper ranaviruses doctor fish virus (DFV), guppy virus 6 (GV6), and largemouth bass virus (LMBV) are members of the genus Ranavirus within the family Iridoviridae. The major capsid protein (MCP) is a main structural protein of iridoviruses and supports the differentiation and classification of ranaviruses. Presently the complete sequence of the MCP gene is known for most ranaviruses with the exception of the Santee-Cooper ranaviruses. In the present study, the complete nucleotide sequence of the MCP gene of DFV, GV6, and LMBV was determined. DFV and GV6 are identical within the MCP gene sequence. The identity compared to the corresponding sequence in LMBV amounts to 99.21%. The MCP gene of DFV, GV6, and LMBV exhibits only approximately 78% identity compared to the respective gene of other ranaviruses. Based on the sequence data obtained in the present study, a Rana MCP polymerase chain reaction (PCR) and subsequent restriction fragment length polymorphism (RFLP) analysis were developed to identify and differentiate ranaviruses, including DFV, GV6, and LMBV.

KEY WORDS: Iridoviridae · Ranavirus · DFV · GV6 · LMBV · EHNV · MCP gene · Rana MCP PCR

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Cite this article as: Ohlemeyer S, Holopainen R, Tapiovaara H, Bergmann SM, Schütze H (2011) Major capsid protein gene sequence analysis of the Santee-Cooper ranaviruses DFV, GV6, and LMBV. Dis Aquat Org 96:195-207

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