DAO 97:1-9 (2011)  -  DOI: https://doi.org/10.3354/dao02396

Polymorphic repetitive loci of the amphibian pathogen Batrachochytrium dendrobatidis

Stephen Garland1,*, Timothy Y. James2, David Blair3, Lee Berger1, Lee F. Skerratt1

1Amphibian Disease Ecology Group, and the School of Public Health, Tropical Medicine and Rehabilitation Sciences, James Cook University, Townsville, Queensland 4811, Australia
2Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, Michigan 48109-1048, USA
3School of Marine and Tropical Biology, James Cook University, Townsville, Queensland 4811, Australia

ABSTRACT: Batrachochytrium dendrobatidis (Bd), the cause of a fatal fungal skin disease of amphibians that has led to massive die-offs, global declines and extinctions, has spread internationally as a pandemic clone with low genetic diversity. A need exists to develop highly polymorphic markers to determine centers of origin and patterns of spread to assist in the development of management strategies. Comparison of paralogous sequences, obtained from the 2 sequenced Bd genomes, indicates useful levels of inter-strain polymorphism in repetitive fragments. We assessed 6 repetitive loci for variation within and among Australian isolates using standard fragment analysis and capillary electrophoresis-single strand conformation polymorphism (CE-SSCP) analysis. Confirmation of inter-isolate polymorphism was achieved for 2 marker systems, highlighting the potential of repetitive loci for the development of polymorphic markers in Bd. In addition, we found that repetitive loci in Bd include possible orthologs of virulence-related genes from pathogenic fungi.


KEY WORDS: Batrachochytrium dendrobatidis · Chytrid · Molecular strain typing · SSCP


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Cite this article as: Garland S, James TY, Blair D, Berger L, Skerratt LF (2011) Polymorphic repetitive loci of the amphibian pathogen Batrachochytrium dendrobatidis. Dis Aquat Org 97:1-9. https://doi.org/10.3354/dao02396

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