DAO 97:227-235 (2012)  -  DOI: https://doi.org/10.3354/dao02422

Azumiobodo hoyamushi gen. nov. et sp. nov. (Euglenozoa, Kinetoplastea, Neobodonida): a pathogenic kinetoplastid causing the soft tunic syndrome in ascidian aquaculture

Euichi Hirose1, Akino Nozawa2,3, Akira Kumagai4, Shin-Ichi Kitamura2,3,*

1Department of Chemistry, Biology and Marine Science, University of the Ryukyus, Nishihara, Okinawa 903-0213, Japan
2Department of Biology, Faculty of Science, Ehime University, Matsuyama, Ehime 790-8577, Japan
3Centre for Marine Environmental Studies (CMES), Ehime University, Matsuyama, Ehime 790-8577, Japan
4Miyagi Prefecture Fisheries Technology Institute, Ishinomaki, Miyagi 986-2135, Japan
*Corresponding author. Email:

ABSTRACT: We used morphological and genetic analyses to investigate a pathogenic kinetoplastid isolated from a diseased edible ascidian Halocynthia roretzi with soft tunic syndrome. The morphological characteristics of the kinetoplastid are similar to those in the order Neobodonida in the subclass Metakinetoplastida. However, the presence of unique globular bodies distinguishes this kinetoplastid from the other polykinetoplastic genera (i.e. Cruzella, Dimastigella and Rhynchobodo) in this order. These globular bodies are cytoplasmic inclusions without an outer delimiting membrane and are composed of a homologous granular matrix containing electron-dense bands. A phylogenetic tree based on 18S rRNA gene sequences also indicated that the kinetoplastid belongs to the order Neobodonida, although it forms an independent clade in this order. From these results, we propose a new genus in the order Neobodonida, i.e. Azumiobodo gen. nov., and Azumiobodo hoyamushi as the type species for the genus.


KEY WORDS: New kinetoplastid · Neobodonida · Azumiobodo · Pathogenic · Soft tunic syndrome · Ascidian aquaculture


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Cite this article as: Hirose E, Nozawa A, Kumagai A, Kitamura SI (2012) Azumiobodo hoyamushi gen. nov. et sp. nov. (Euglenozoa, Kinetoplastea, Neobodonida): a pathogenic kinetoplastid causing the soft tunic syndrome in ascidian aquaculture. Dis Aquat Org 97:227-235. https://doi.org/10.3354/dao02422

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