DAO 98:255-259 (2012)  -  DOI: https://doi.org/10.3354/dao02445

NOTE
PCR detection of the crayfish plague pathogen in narrow-clawed crayfish inhabiting Lake Eğirdir in Turkey

J. Svoboda1, E. Kozubíková1, P. Kozák2, A. Kouba2, S. Bahadir Koca3, Ö. Diler3, I. Diler3, T. Policar2, A. Petrusek1,*

1Department of Ecology, Faculty of Science, Charles University in Prague, 128 44 Prague 2, Czech Republic
2Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses and Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in České Budějovice, 389 25 Vodň any, Czech Republic
3Eg˘irdir Fisheries Faculty, Süleyman Demirel University, 32 500 Eg˘irdir, Isparta, Turkey

ABSTRACT: Many populations of the narrow-clawed crayfish Astacus leptodactylus in Turkey, including those inhabiting Lake Eğirdir, declined drastically in the mid-1980s due to introduction of crayfish plague Aphanomyces astaci. However, unlike many other localities, there has been some recovery in the A. leptodactylus population inhabiting this lake even though crayfish plague has been suspected to have persisted since then. In support of this, DNA from 5 of 34 healthy-looking crayfish sampled recently from the lake tested positive by both conventional and real-time PCR using species-specific primers targeting the rDNA internal transcribed spacer region, and product sequence analysis confirmed the identification of A. astaci. This complies with other recent reports of coexistence of native European crayfish with this pathogen, and further research is now needed to identify the key mechanisms allowing it.


KEY WORDS: Aphanomyces astaci · Astacus leptodactylus · Host−pathogen coexistence · Melanisation · rDNA-internal transcribed spacer sequence · Lake Eğirdir


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Cite this article as: Svoboda J, Kozubíková E, Kozák P, Kouba A and others (2012) PCR detection of the crayfish plague pathogen in narrow-clawed crayfish inhabiting Lake Eğirdir in Turkey. Dis Aquat Org 98:255-259. https://doi.org/10.3354/dao02445

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