DAO 99:153-162 (2012)  -  DOI: https://doi.org/10.3354/dao02463

Indel-II region deletion sizes in the white spot syndrome virus genome correlate with shrimp disease outbreaks in southern Vietnam

Tran Thi Tuyet Hoa1,2,3, Mark P. Zwart4, Nguyen T. Phuong2, Dang T. H. Oanh2, Mart C. M. de Jong3, Just M. Vlak1,*

1Laboratory of Virology, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands
2College of Aquaculture and Fisheries, Can Tho University, Campus II, 3/2 street, Can Tho City, Vietnam
3Quantitative Veterinary Epidemiology, Wageningen University, Marijkeweg 40, 6709 PG Wageningen, The Netherlands
4Instituto de BiologĂ­a Molecular y Celular de Plantas, Consejo Superior de Investigaciones CientĂ­ficas-UPV, Valencia, Spain

ABSTRACT: Sequence comparisons of the genomes of white spot syndrome virus (WSSV) strains have identified regions containing variable-length insertions/deletions (i.e. indels). Indel-I and Indel-II, positioned between open reading frames (ORFs) 14/15 and 23/24, respectively, are the largest and the most variable. Here we examined the nature of these 2 indel regions in 313 WSSV-infected Penaeus monodon shrimp collected between 2006 and 2009 from 76 aquaculture ponds in the Mekong Delta region of Vietnam. In the Indel-I region, 2 WSSV genotypes with deletions of either 5950 or 6031 bp in length compared with that of a reference strain from Thailand (WSSV-TH-96-II) were detected. In the Indel-II region, 4 WSSV genotypes with deletions of 8539, 10970, 11049 or 11866 bp in length compared with that of a reference strain from Taiwan (WSSV-TW) were detected, and the 8539 and 10970 bp genotypes predominated. Indel-II variants with longer deletions were found to correlate statistically with WSSV-diseased shrimp originating from more intensive farming systems. Like Indel-I lengths, Indel-II lengths also varied based on the Mekong Delta province from which farmed shrimp were collected.


KEY WORDS: White spot syndrome virus · Disease status · Farming system · Epidemiology · ORF 14/15 · ORF 23/24


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Cite this article as: Hoa TTT, Zwart MP, Phuong NT, Oanh DTH, de Jong MCM, Vlak JM (2012) Indel-II region deletion sizes in the white spot syndrome virus genome correlate with shrimp disease outbreaks in southern Vietnam. Dis Aquat Org 99:153-162. https://doi.org/10.3354/dao02463

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