Inter-Research > MEPS > v511 > p41-58  
MEPS
Marine Ecology Progress Series

via Mailchimp

MEPS 511:41-58 (2014)  -  DOI: https://doi.org/10.3354/meps10933

Spatio-temporal variability in ammonia oxidation and ammonia-oxidising bacteria and archaea in coastal sediments of the western English Channel

K. Tait1,*, V. Kitidis1, B. B. Ward2, D. G. Cummings1, M. R. Jones1, P. J. Somerfield1, S. Widdicombe1

1Plymouth Marine Laboratory, Prospect Place, Plymouth, PL1 3DH, UK
2Department of Geosciences, Guyot Hall, Princeton University, Princeton, NJ 08544, USA
*Corresponding author:

ABSTRACT: The abundance of ammonia-oxidising bacterial (AOB) and ammonia-oxidising archaeal (AOA) (amoA) genes and ammonia oxidation rates were compared bimonthly from July 2008 to May 2011 in 4 contrasting coastal sediments in the western English Channel. Despite a higher abundance of AOA amoA genes within all sediments and at all time-points, rates of ammonia oxidation correlated with AOB and not AOA amoA gene abundance. Sediment type was a major factor in determining both AOB amoA gene abundance and AOB community structure, possibly due to deeper oxygen penetration into the sandier sediments, increasing the area available for ammonia oxidation. Decreases in AOB amoA gene abundance were evident during summer and autumn, with maximum abundance and ammonia oxidation rates occurring in winter and early spring. PCR-DGGE of AOB amoA genes indicated that no seasonal changes to community composition occurred; however, a gradual movement in community composition occurred at 3 of the sites studied. The lack of correlation between AOA amoA gene abundance and ammonium oxidation rates, or any other environmental variable measured, may be related to the higher spatial variation amongst measurements, obscuring temporal trends, or the bimonthly sampling, which may have been too infrequent to capture temporal variability in the deposition of fresh organic matter. Alternatively, AOA may respond to changing substrate concentrations by an increase or decrease in transcript rather than gene abundance.


KEY WORDS: Coastal · Sediment · Ammonia oxidation · Archaeal amoA gene · Bacteria amoA gene


Full text in pdf format 
Cite this article as: Tait K, Kitidis V, Ward BB, Cummings DG, Jones MR, Somerfield PJ, Widdicombe S (2014) Spatio-temporal variability in ammonia oxidation and ammonia-oxidising bacteria and archaea in coastal sediments of the western English Channel. Mar Ecol Prog Ser 511:41-58. https://doi.org/10.3354/meps10933

Export citation
Mail this link - Contents Mailing Lists - RSS
Facebook - - linkedIn