MEPS 544:143-158 (2016)  -  DOI: https://doi.org/10.3354/meps11591

Effects of elevated pCO2 and irradiance on growth, photosynthesis and calcification in Halimeda discoidea

K. E. Peach1,*, M. S. Koch1, P. L. Blackwelder2

1Florida Atlantic University, Department of Biological Sciences, 777 Glades Road, Boca Raton, FL 33431, USA
2Nova Southeastern University, 8000 North Ocean Drive, Dania Beach, FL 33004 and University of Miami, Center for Advanced Microscopy, 1252 Memorial Drive, Coral Gables, FL 33146, USA
*Corresponding author:

ABSTRACT: Ocean acidification (OA) effects on photophysiology and calcification were examined in Halimeda discoidea, a calcifying macroalga that produces tropical reef sediments. Photosynthetic parameters, including maximum photosynthetic rate (Pmax), photosynthetic efficiency (α) and compensating irradiance (Ic) were determined in short-term assays on live thalli after a 10 d exposure to 4 levels of CO2 partial pressures ( pCO2; 491, 653, 982 and 1201 µatm) under saturating (300 µmol photons m-2 s-1) and sub-saturating (90 µmol photons m-2 s-1) irradiance in an aquaria study. Morphology of aragonite crystals produced in segments formed on adult thalli was characterized using scanning electron microscopy (SEM). Further, we examined crystal morphology and changes in inorganic content of non-living segments exposed to elevated (1201 µatm) and ambient pCO2 for 27 d to assess OA effects on carbonate sediments generated from H. discoidea. Even though Pmax was higher under elevated pCO2, this photophysiological response did not result in higher calcification rates. Based on crystal measurements and SEM imagery, aragonite crystals within new segments were indistinguishable across pCO2 and irradiance treatments. Under high irradiance, new segments showed a greater investment in organic versus inorganic production. Non-living segments contained narrower crystals after 27 d exposure to elevated pCO2 relative to controls, but differences were small (0.03 µm) and did not contribute significantly to changes in normalized biomass or inorganic content. Based on these results, H. discoidea will likely produce new calcified segments with intact aragonite crystals under year 2100 pCO2 levels at high and low irradiance, while aragonite crystals of the sediment may produce thinner needle carbonate muds.


KEY WORDS: Photosynthesis · Calcification · Dissolution · CO2 · Ocean acidification · Irradiance · Halimeda


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Cite this article as: Peach KE, Koch MS, Blackwelder PL (2016) Effects of elevated pCO2 and irradiance on growth, photosynthesis and calcification in Halimeda discoidea. Mar Ecol Prog Ser 544:143-158. https://doi.org/10.3354/meps11591

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