DAO 94:59-72 Supplementary Download

CONT-1-attaching
The parasite can be seen on a fragment of kidney, moving from one locations to another by attaching and releasing with its anterior prohaptor and posterior haptor.

CONT-2-stretching-rotating
Three parasites in well, prior to adding drugs. Their motion is typical of control parasites, relaxation, contraction and twisting around the longitudinal axis.

CONT-3-rotating-sucker
Normal activity of the anterior prohaptor.

CONT-4-rotating-haptor
Normal activity of the haptor.

CONT-5-haptor-hooklets
The hooklets in the haptor move actively and separately.

CONT-6-egg-repro tract
Two parasites in tissue. One is moving, with an egg inside female reproductive system of the adult parasite.

CONT-7-egg-embryo
The embryo, with prominent eye spots, is actively moving within the egg. The embryo has made rapidly moving cilia.

CONT-8-hatchling
Ciliated hatchling (oncomiracidium) travels rapidly, occasionally spiraling up and down in the water column.

RX-1-reduced motion-less stretching
First video clip shows normal motion immediately after drug administration, second shows subsequent slowing and reduced relaxing and contracting.

RX-2-reduced motion-ends bend
This parasite continues to slowly move the haptor and prohaptor, but the mid-portion of body is immobile of slightly contracted.

RX-3-contracted-minor body stretch
This worm remains contracted, with occasional slight jerky elongation at the anterior end.

RX-4-contracted-minimal hooklet motion
These worms initially appear to be imobile, but the middle one is slowly and moving several hooklets.

RX-5-contracted-tremors 2 near dead
First video clip shows 3 parasites, right parasite is dead, top is barely moving, and lower parasite has slowed motion with intense tremors after contracting. Second video clip is a parasite with tremors.

RX-6-reduced motion-segmented
Segmented body appearance, with peristaltic type contractions along the mid portion of the body. This appearance wa s only rarely seen.