DAO prepress abstract  -  DOI: https://doi.org/10.3354/dao03180

Evaluation of blue mussel Mytilus edulis as vector for viral hemorrhagic septicemia virus

Kwang Il Kim, Young Chul Kim, Woo Ju Kwon, Hyun Do Jeong*

*Email: jeonghd@pknu.ac.kr

ABSTRACT: When viral diseases occur in aquaculture farms, the virus released into seawater from infected animals can re-infect other susceptible species or accumulate in filter-feeding organisms. We conducted a viral hemorrhagic septicemia virus (VHSV) survivability analysis of blue mussel (Mytilus edulis) digestive enzymes, viral depuration, and infectivity tests via in vitro and in vivo inoculation to evaluate the infectious state. VHSV particles were not completely digested within 24 h in vitro and were maintained for 7 d in the mussel digestive gland. Mussels cohabitating with naturally VHSV-infected olive flounder (Paralichthys olivaceus) could accumulate the viral particles. Although the viral particles in the gill as entrance of filter-feeding organisms are infectious, the presence of these particles in digestive gland were not able to induce in vitro. Viral particles detected by RT-PCR from bivalve mollusks (Pacific oyster, Craassostrea gigas and mussel) in the field did not produce CPE in cell culture and did not replicate after intraperitioneal injection into olive flounder. Therefore, VHSV particles in blue mussel might be in a non-infectious stage and the possibilities of VHSV transmission to fish under field conditions are scarce.