AB 24:17-24 (2015)  -  DOI: https://doi.org/10.3354/ab00632

Development of molecular resources for the Chinese horseshoe crab Tachypleus tridentatus

Hongjun Li1, Ying Xia2, Yiyong Rao4, Shuxi Liu1, Jingjing Zhang1,5, Lizhe Cai3,4,*

1Key Laboratory for Ecological Environment in Coastal Areas (SOA), National Marine Environmental Monitoring Center, Dalian 116023, PR China
2Dalian Environmental Monitoring Center, Dalian 116023, PR China
3Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, Xiamen University, Xiamen 361102, PR China
4College of the Environment and Ecology, Xiamen University, Xiamen 361002, PR China
5Dalian Ocean University, Dalian 116023, PR China
*Corresponding author:

ABSTRACT: The Chinese horseshoe crab Tachypleus tridentatus is an endangered marine benthic species of great biomedical value and scientific significance. In this study, T. tridentatus hemocyte transcriptome was sequenced to rapidly develop molecular resources using the Illumina paired-end sequencing platform. Deep sequencing generated a total of 38402764 reads that consisted of 7.7 Gbp raw data, which were assembled into 38759 unigenes with an average length of 668.6 bp. A total of 608 microsatellite loci and 71959 high-confidence single nucleotide polymorphisms were discovered. All the assembled unigenes were annotated by running BLASTx similarity searches on Nr, Swiss-Prot, COG, and KEGG databases; of which 17446 (45.0%) unigenes showed significant matches (E-value <1e-5) to known sequences in public databases. As determined through gene ontology annotation and KEGG pathway mapping, the functional annotation of the unigenes signified diverse biological functions and processes. Transcripts potentially involved in immune defense were identified among these genes. These transcripts included pattern recognition receptors, stress response genes, complement components, and immune effectors. This study provides  important insights into the transcript sequences of T. tridentatus, which may facilitate studies on the conservation genetics and development of biomedical applications for T. tridentatus.

KEY WORDS: Chinese horseshoe crab · Tachypleus tridentatus · Endangered species · Transcriptome · RNA-seq · Microsatellite · SNP · Conservation genomics · Biomedicine

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Cite this article as: Li H, Xia Y, Rao Y, Liu S, Zhang J, Cai L (2015) Development of molecular resources for the Chinese horseshoe crab Tachypleus tridentatus. Aquat Biol 24:17-24. https://doi.org/10.3354/ab00632

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