DOI: https://doi.org/10.3354/ab00675
copiedAssignment of parentage by microsatellite analysis in the endangered Brachymystax lenok tsinlingensis (Salmonidae)
ABSTRACT: We evaluated the use of microsatellite markers to identify parentage in Brachymystax lenok tsinlingensis Li, 1966, an endangered salmonid. Computer simulations showed that when no parent information was available, or when information was only available from 1 parent, then exclusion probabilities ranged from 23.6 to 45.1% and from 25.9 to 52.4%, respectively; combined exclusion probabilities for the 8 loci investigated were 97.7 and 98.4%, respectively. However, a breeding experiment with known parental and filial information resulted in 91.28% of progeny being exclusively assigned to their correct parent pair. Our data demonstrate that microsatellite-based parentage assignment is a reliable means with which to obtain information pertaining to genealogical relationships and could therefore benefit genetic conservation of this species.
KEYWORDS
Jian Shao (Co-author)
- College of Fisheries, Huazhong Agricultural University, Wuhan 430070, PR China
- Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, PR China
- College of Animal Science, Guizhou University, GuiYang 550025, PR China
Wei Luo (Co-author)
- South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, PR China
Qiwei Wei (Corresponding Author)
- College of Fisheries, Huazhong Agricultural University, Wuhan 430070, PR China
- Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, PR China
Feng Wang (Co-author)
- Yellow River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Xi’an 710086, PR China
Wei Guo (Co-author)
- Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, PR China
Huan Ye (Co-author)
- Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, PR China
Zhipeng Chu (Co-author)
- Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, PR China
Jinming Wu (Co-author)
- Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, PR China
Shuhuan Zhang (Co-author)
