AB 26:169-178 (2017)  -  DOI: https://doi.org/10.3354/ab00684

Significance of HCO3- alkalinity in calcification and utilization of dissolved inorganic carbon in Chara vulgaris 

Heyun Wang1,2, Leyi Ni2,*, Dan Yu

1College of Resource and Environmental Engineering, Hubei University of Technology, Wuhan 430068, PR China
2Donghu Experimental Station of Lake Ecosystems, State Key Laboratory for Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, The Chinese Academy of Sciences, Donghu South Road 7, Wuhan 430072, PR China
3Freshwater National Observation and Research Station of Lake Ecosystem in Liangzi Lake, College of Life Sciences, Wuhan University, Wuhan 430072, PR China
*Corresponding author:

ABSTRACT: To investigate the influence of HCO3- alkalinity on calcification in thalli of the genus Chara, we studied the effects of increased light level and additional HCO3- on calcification rate in C. vulgaris at various hours (30 min, 8 h, 12 h, 12.5 h, 20 h and 24 h after treatment) in a 24 h experiment (12 h light:12 h dark). We identified a significant Pearson’s correlation between exogenous dissolved inorganic carbon (DIC) concentrations and the utilization of DIC (µmol C g-1 fresh weight). Plotting the daily rhythm of DIC utilization produced negative quadratic curves. Furthermore, calcification rate (µmol Ca h-1 g-1) was linearly related to DIC utilization rate (DICuptake; µmol C h-1 g -1), indicating that the calcification rate is dependent on DICuptake. However, ratios of calcification to the utilization of DIC were decreased at high light intensity and increased with HCO3- addition, which was mainly ascribed to changes in the ratio of calcification to photosynthesis. Chlorophyll fluorescence results provided direct evidence for the promotion of photosynthesis in Chara thalli by both high light and DIC addition and their positive influence on maximum relative electron transport rate. These results suggest that calcification in calcareous C. vulgaris is mainly restrained by HCO3- alkalinity, which could explain the correlation between calcification of Chara thalli and alkalinity of water bodies in the field.

KEY WORDS: Calcification · Dissolved inorganic carbon utilization · Rapid light curves · Chara · Calcification · Photosynthesis

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Cite this article as: Wang H, Ni L, Yu D (2017) Significance of HCO3- alkalinity in calcification and utilization of dissolved inorganic carbon in Chara vulgaris . Aquat Biol 26:169-178. https://doi.org/10.3354/ab00684

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