AB 26:75-85 (2017)  -  DOI: https://doi.org/10.3354/ab00674

Acute sulphide toxicity in Perinereis aibuhitensis under different salinities and temperatures: LC50 and antioxidant responses

Haiqing Wang1,2, Guodong Wang3, Jianguang Fang1,4, Zengjie Jiang1,4, Meirong Du1,4, Yaping Gao1,4, Jinghui Fang1,4,*

1Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, 266071 Qingdao, PR China
2Norwegian University of Science and Technology, 7491 Trondheim, Norway
3Marine Science and Engineering College, Qingdao Agriculture University, 266109 Qingdao, PR China
4Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, 266237 Qingdao, PR China
*Corresponding author:

ABSTRACT: Sulphide accumulates in sediment due to global aquaculture development and is very harmful to aquatic and benthic ecosystems. Perinereis aibuhitensis inhabits coastal areas and is often exposed to fairly high sulphide concentrations. The present study investigated the tolerance of P. aibuhitensis to sulphide to evaluate its potential application in sulphide remediation and sustainable aquaculture. We assessed the toxicity of sulphide on P. aibuhitensis in a 96 h acute sulphide exposure experiment under different temperature and salinity conditions. Two-way ANOVA showed that increasing salinity did not influence the LC50 of P. aibuhitensis exposed to sulphide. In contrast, increasing temperature significantly augmented the LC50 value (p < 0.05). The results showed a negative relationship between mortality and temperature, and between mortality and exposure time. Subsequently, we performed 2-way ANOVA analysis of the antioxidant (superoxide dismutase [SOD], catalase [CAT] and total antioxidant capacity [T-AOC]) responses of P. aibuhitensis during 96 h exposure to sub-lethal sulphide concentrations (0, 80, 160, 320, and 640 ┬Ámol l-1), and a subsequent recovery period. The activation of this antioxidant defense system appeared to depend on sulphide concentration and exposure duration and their interaction. SOD, CAT, and T‑AOC showed obvious differences at the beginning and end of exposure. They were steadily restored during the recovery period. The results indicated that P. aibuhitensis adjusts its antioxidant defense system to cope with sulphide contamination. Therefore, these indexes of P. aibuhitensis could be applied to environmental monitoring and bio-restoration at mudflat or intensive aquaculture areas with high sulphide concentrations.

KEY WORDS: Sulphide · Perinereis aibuhitensis · LC50 · Oxidative stress · Antioxidant enzyme

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Cite this article as: Wang H, Wang G, Fang J, Jiang Z, Du M, Gao Y, Fang J (2017) Acute sulphide toxicity in Perinereis aibuhitensis under different salinities and temperatures: LC50 and antioxidant responses. Aquat Biol 26:75-85. https://doi.org/10.3354/ab00674

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