Inter-Research > AB > v29 > p59-70  
AB
Aquatic Biology

via Mailchimp

AB 29:59-70 (2020)  -  DOI: https://doi.org/10.3354/ab00725

Comparison of growth and toxicity responses between non-toxic and toxic strains of Prorocentrum hoffmannianum

Thomas Chun-Hung Lee*, Ping-Lung Chan*, Steven Jingliang Xu, Fred Wang-Fat Lee**

Department of Science, School of Science and Technology, The Open University of Hong Kong, Hong Kong SAR
*These co-first authors contributed equally**Corresponding author:

ABSTRACT: We aimed to study the growth and toxicity responses of non-toxic (CCMP683) and toxic (CCMP2804) strains of Prorocentrum hoffmannianum under various nitrate and phosphate concentrations. The 2 strains were cultured in L1-Si medium with standard, depleted or 10-fold repleted nitrate or phosphate. CCMP683 cultured in standard L1-Si medium exhibited delayed growth. Nitrate or phosphate depletion decreased the cell density of both strains. Repletion of nitrate slightly increased the cell density of both strains. Repletion of phosphate also slightly increased the cell density of CCMP2804 but surprisingly decreased the cell density of CCMP683. Okadaic acid (OA) and its derivatives were not detected in CCMP683. OA was detected only in CCMP2804. Depletion of nitrate or phosphate increased the cellular concentration of OA, and repletion of nitrate or phosphate had no effect on the cellular concentration of OA. Correlation analysis indicated that the cellular concentration of OA was negatively correlated with cell density. Differences in the growth response to phosphate repletion and in the ability to produce OA suggest that the 2 strains may be good candidates for comparative studies related to phosphate metabolism and OA toxicity.


KEY WORDS: Prorocentrum hoffmannianum · Dinoflagellates · Okadaic acid · Diarrhetic shellfish poisoning · DSP · Harmful algae · Nitrate · Phosphate · Cell cycle


Full text in pdf format 
Cite this article as: Lee TCH, Chan PL, Xu SJL, Lee FWF (2020) Comparison of growth and toxicity responses between non-toxic and toxic strains of Prorocentrum hoffmannianum. Aquat Biol 29:59-70. https://doi.org/10.3354/ab00725

Export citation
Mail this link - Contents Mailing Lists - RSS
Facebook - - linkedIn