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Aquaculture Environment Interactions

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AEI 11:75-86 (2019)  -  DOI:

In vivo DNA damage in gill, haemolymph and muscle cells of whiteleg shrimp Litopenaeus vannamei on exposure to organophosphorus pesticide

Ashwini P. Pawar1,2, Sushant V. Sanaye1, Soorambail Shyama2, Rayadurga A. Sreepada1,*, Jacky Bhagat2, Praveen Kumar2, Rakhee D. Sinai Khandeparker1

1Aquaculture Laboratory, Biological Oceanography Division, CSIR-National Institute of Oceanography, Goa 403 004, India
2Department of Zoology, Goa University, Taleigao Plateau, Goa 403 206, India
*Corresponding author:

ABSTRACT: In response to growing worldwide market demand, intensive shrimp farming, particularly of whiteleg shrimp Litopenaeus vannamei, has expanded tremendously. The present study investigated induced DNA damage in gill (GL), haemolymph (HL) and muscle (ML) cells in juveniles of L. vannamei (length: 52.2 ± 6.4 mm; weight: 1.78 ± 0.5 g; mean ± SD) exposed to 2 sublethal (SL) concentrations, SL1 (0.36 µg l-1) and SL2 (0.18 µg l-1), of the organophosphorus pesticide chlorpyrifos (CPF) during 21 d of exposure (DoE). The magnitude of DNA damage (% tail DNA) as measured by the comet assay at specified intervals (3, 7, 14 and 21 DoE) was found to be tissue specific and time and dose dependent (p < 0.05). At the end of the experiment, at 21 DoE, % tail DNA damage was relatively higher at SL1 (53.61 ± 8.71, 49.36 ± 3.42 and 32.40 ± 4.97%) compared to SL2 (39.25 ± 3.90, 32.22 ± 4.21 and 22.66 ± 2.85%) in GL, HL and ML cells, respectively. No significant differences in water quality parameters were found among treated and control aquaria. The significant reduction in specific growth rates (% growth d-1) observed in exposed shrimps indicated that SL concentrations of CPF negatively impacted growth in L. vannamei juveniles. A very low 96 h median lethal concentration (1.44 µg l-1) indicated sensitivity of L. vannamei juveniles to CPF, suggesting that the species could be used as a bioindicator for assessing pesticide pollution. The study results highlight the implications of extending the farming of L. vannamei to low-salinity inland areas adjacent to traditional agricultural fields.

KEY WORDS: Chlorpyrifos · Organophosphorus pesticide · DNA damage · Comet assay · Whiteleg shrimp · Litopenaeus vannamei

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Cite this article as: Pawar AP, Sanaye SV, Shyama S, Sreepada RA, Bhagat J, Kumar P, Khandeparker RDS (2019) In vivo DNA damage in gill, haemolymph and muscle cells of whiteleg shrimp Litopenaeus vannamei on exposure to organophosphorus pesticide. Aquacult Environ Interact 11:75-86.

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