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Aquaculture Environment Interactions

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AEI 9:21-32 (2017)  -  DOI:

Responses in bacterial community structure to waste nutrients from aquaculture: an in situ microcosm experiment in a Chilean fjord

Lasse Mork Olsen1,*, Klaudia L. Hernández2, Murat Van Ardelan3, Jose Luis Iriarte4,5, K. Can Bizsel6, Yngvar Olsen

1Norwegian Polar Institute, Fram Centre, 9296 Tromsø, Norway
2Facultad de Ecologia y Recursos Naturales, Universidad Andres Bello, 2520000 Viña del Mar, Chile
3Norwegian University of Science and Technology, Dept. of Chemistry, 7491 Trondheim, Norway
4Instituto de Acuicultura and Centro FONDAP de Investigación Dinámica de Ecosistemas Marinos de Altas Latitudes (IDEAL), Universidad Austral de Chile, 5480000 Puerto Montt, Chile
5COPAS-Sur Austral, Centro de Investigación Oceanográfica en el Pacífico Sur-Oriental (COPAS), Universidad de Concepción, 4030000 Concepción, Chile
6Dokuz Eylül University, Institute of Marine Sciences and Technology, Bakü Bulv. No. 100, 35340 Inciralt, Izmir, Turkey
7Norwegian University of Science and Technology, Dept. of Biology, 7491 Trondheim, Norway
*Corresponding author:

ABSTRACT: Chilean salmon farms release inorganic nutrients excreted by the fish into the surrounding water in Patagonian fjords. The objective of this experiment from the Comau Fjord (42.2° S) in southern Chile was to study how increased input of ammonium (NH4) and phosphate (PO4) from salmon farms might affect the community structure of bacteria in surface waters where fish farms are located. We used microcosms (35 l) with NH4-N and PO4-P added to the natural seawater in a gradient of nutrient-loading rates, with the same N:P ratio as in salmon aquaculture effluents. Additionally, we measured bacterial community structure at different depths in the Comau Fjord to assess the natural variation to compare with our experiment. We used denaturing gradient gel electrophoresis (DGGE) to create 16S rDNA fingerprints of the bacterial communities and monitored biological and environmental variables (chlorophyll a, inorganic nutrients, pH, microbial abundance). The nutrient-loading rate had a significant impact on the bacterial community structure, and the community dissimilarity between low and high nutrient additions was up to 78%. Of the measured environmental variables, phytoplankton abundance and increased pH from photosynthesis had a significant effect. We observed no significant changes in bacterial diversity, which remained at the same level as in the unmanipulated community. Thus, the bacterial community of the fjord was not resistant, but resilient within the time frame and nutrient gradient of our experiment.

KEY WORDS: Aquaculture · Eutrophication · Microbial community · Bacterial diversity · Chile · Patagonia

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Cite this article as: Olsen LM, Hernández KL, Ardelan MV, Iriarte JL, Bizsel KC, Olsen Y (2017) Responses in bacterial community structure to waste nutrients from aquaculture: an in situ microcosm experiment in a Chilean fjord. Aquacult Environ Interact 9:21-32.

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