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Aquatic Microbial Ecology

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AME 15:39-51 (1998)  -  doi:10.3354/ame015039

Seawater microorganisms have a high affinity glycine betaine uptake system which also recognizes dimethylsulfoniopropionate

Ronald P. Kiene1,2,*, Lynn P. Hoffmann Williams1,2, Joel E. Walker1,2

1Department of Marine Sciences, University of South Alabama, Mobile, Alabama 36688, USA 2Dauphin Island Sea Lab, Dauphin Island, Alabama 36528, USA

In 24 h old seawater filtrate cultures, comprised mostly of free-living bacteria, the uptake of methyl-14C-glycine betaine (GBT) displayed Michaelis-Menten-type saturation kinetics with half-saturation constants (Kt) of <5 nM. The uptake of 5 nM 14C-GBT was strongly inhibited by additions of 1 to 30 nM unlabeled b-dimethylsulfoniopropionate (DMSP), a naturally occurring analog of GBT. The dose response to DMSP was consistent with simple dilution of labeled GBT. Conversely, the uptake of 35S-DMSP was inhibited by the presence of GBT over a similar concentration range. Comparison of 14C-GBT and 35S-DMSP uptake kinetics in the presence and absence of unlabeled analogs suggested that GBT and DMSP act as competitive inhibitors of their respective uptake by seawater microbes. Direct comparisons of 14C-GBT and 35S-DMSP uptake in filtrate cultures yielded nearly identical kinetic patterns. It was also found that a portion of the 14C-GBT taken up into particulate material could be chased from the particles by a 200-fold excess of unlabeled GBT or DMSP, whereas it was retained as untransformed 14C-GBT in the particles for several hours in unchased samples. A screening of organic compounds revealed that compounds with close structural similarity to GBT (DMSP, dimethylsulfonioacetate, proline betaine and dimethylglycine) strongly inhibited (<36% of controls) 14C-GBT uptake. Compounds showing moderate inhibition (48 to 89% of controls) included trigonelline, proline, glutamic acid, carnitine and choline, while compounds bearing no structural or chemical similarity to GBT (glycine, glucose, acrylic acid, and 3-methiolpropionate) had no effects on 14C-GBT uptake. Our results indicate that the GBT uptake system expressed by natural populations of microorganisms is multifunctional, displaying high affinity for both GBT and DMSP and possibly other naturally occurring betaine-like compounds. This microbial uptake system is likely to play an important role in the biogeochemical dynamics of GBT and DMSP.

Osmolytes · Transport · Bacteria · Dimethylsulfoniopropionate · Inhibitors

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