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Aquatic Microbial Ecology

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AME 17:105-110 (1999)  -  doi:10.3354/ame017105

Impact of acetate, pyruvate, and physiological state on respiration and respiratory quotients in Pseudomonas nautica

Sylvie O. Roy1,*, Ted T. Packard2, Elisa Berdalet2, Liliane St-Amand1

1Institut Maurice-Lamontagne, Pêches et Océans, CP 1000, Mont-Joli, Québec G5H 3Z4, Canada
2Institut de Ciències del mar (CSIC), P. Joan de Borbó, s/n, E-08039 Barcelona, Spain

ABSTRACT: Physiological rates of CO2 production and O2 consumption, and the activities of isocitrate dehydrogenase (IDH) and the electron transfer system (ETS) were studied in the marine bacterium Pseudomonas nautica growing on acetate. In exponential growth, IDH and ETS activities were well coupled with CO2 production and O2 consumption rates, but in senescence they were uncoupled. Our results clearly show that under starvation conditions, IDH and ETS activities remained high even though their corresponding respiration rates dropped. The respiratory metabolism in the different physiological states of the acetate-grown cultures was compared with previous observations made in pyruvate-grown cultures. Time profiles of CO2 production and O2 consumption rates showed completely different respiratory fingerprints associated with the different carbon sources. Acetate-grown cultures showed an increase of respiratory quotients (RQ) in the senescence phase whereas in pyruvate-grown cultures it stayed close to 1.0. On both carbon sources, respiration to respiratory capacity ratios were constant in exponential phase and decreased to almost zero after carbon source exhaustion. Our results clearly show the impact of physiological state and carbon sources on bacterial respiration rates.

KEY WORDS: CO2 production rates · O2 consumption rates · Isocitrate dehydrogenase · Respiratory electron transfer system · ETS · Marine bacterium

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