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Aquatic Microbial Ecology

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AME 43:267-276 (2006)  -  doi:10.3354/ame043267

Pressure effects on surface Mediterranean prokaryotes and biogenic silica dissolution during a diatom sinking experiment

Christian Tamburini1,*, Jean Garcin1, Gérald Grégori1, Karine Leblanc2, Peggy Rimmelin2, David L. Kirchman3

1Laboratoire Microbiologie, Géochimie et Ecologie Marines, UMR 6117 CNRS—INSU, and
2Laboratoire d’Océanographie et de Biogéochimie, UMR 6535 CNRS—INSU, Université de la Méditerranée, Centre d’Océanologie de Marseille, Campus de Luminy, Case 901, 13288 Marseille, Cedex 9, France
3College of Marine Studies, University of Delaware, Lewes, Delaware 19958, USA

ABSTRACT: This study examined the effect of increasing pressure on biogenic silica dissolution and on prokaryotic assemblages associated with diatom detritus. Experiments were carried out in hyperbaric bottles subjected to a gradual increase in pressure and compared to incubations at atmospheric pressure. To examine only pressure effects and to simulate detritus degradation in the Mediterranean Sea, the incubation temperature was kept constant (13°C), while pressure was increased by 1.5 MPa d–1, simulating a fall of particles at a sinking rate of 150 m d–1 over 8 d. Aminopeptidase activity was significantly lower (nearly 5-fold) under increasing pressure than under atmospheric pressure conditions. Lower aminopeptidase activity under increasing pressure affected biogenic silica dissolution, at least at the beginning of the incubation, corresponding to a simulated depth of the first 800 m of the water column. Silicic acid regeneration rates were very low (0.07 ± 0.02 µmol l–1 h–1) under increasing pressure conditions during the first 4 d (i.e. between 200 and 800 m), while rates were much higher under atmospheric pressure (0.32 ± 0.05 µmol l–1 h–1). However, orthosilicic acid concentrations in the incubations under increasing pressure approached those of the atmospheric pressure incubations by the end of the experiment. In contrast, the taxonomic composition of prokaryotic communities was not affected by increasing pressure, but the input of fresh diatom detritus led to an increase in the relative abundance of the Cytophago–Flavobacter cluster and γ-Proteobacteria. These results suggest that hydrostatic pressure affects the function rather than the broad taxonomic structure of prokaryotic communities associated with sinking detrital particles.

KEY WORDS: Hydrostatic pressure · Particle sinking · Decomposition processes · Ectoenzymatic activity · Silica dissolution · Prokaryotic diversity · Mesopelagic waters · Bathypelagic waters

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