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Aquatic Microbial Ecology

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AME 47:141-151 (2007)  -  doi:10.3354/ame047141

Bacterial diversity and community structure in acid mine drainage from Dabaoshan Mine, China

Yu Yang1,2, Min-xi Wan1, Wu-yang Shi1, Hong Peng1, Guan-zhou Qiu1,2, Ji-zhong Zhou3, Xue-duan Liu1,2,*

1School of Minerals Processing and Bioengineering, Central South University, 25 South Lushan Road, Changsha, Hunan 410083, China
2Key Laboratory of Biometallurgy, Ministry of Education, 25 South Lushan Road, Changsha, Hunan 410083, China
3Department of Botany and Microbiology, 770 Van Vleet Oval, University of Oklahoma, Norman, Oklahoma 73019-4110, USA

ABSTRACT: Three samples of acid mine drainage (AMD) collected from Dabaoshan Mine (Guangdong Province, China) were studied. In addition to physico-chemical analyses, bacterial diversities and community structures of these samples were described at genetic level by amplified ribosomal DNA restriction analysis (ARDRA). A total of 60 different ARDRA patterns were obtained from 377 clones and were studied as operational taxonomic units (OTUs), which were re-amplified and sequenced. The sequence data and phylogenetic analysis showed that Acidithiobacillus ferrooxidans represented 88.0% of the bacterial population in Sample LC. However, Samples JX and FS contained more diverse colonies of bacteria, such as Leptospirillum ferrooxidans (JX: 16.9%, FS: 39.1%), Acidiphilum sp. (JX: 38.7%, FS: 25.8%) and A. ferrooxidans (JX: 12.1%, FS: 10.2%). These diversities were characterized by the reciprocal of Simpson’s index (1/D) and correlated with the concentrations of ferrous iron and toxic ions in AMD.

KEY WORDS: Bacterial diversity · Microbial community · Acid mine drainage · Amplified ribosomal DNA restriction analysis

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