Inter-Research > AME > v49 > n3 > p233-241  
Aquatic Microbial Ecology

via Mailchimp

AME 49:233-241 (2007)  -  DOI:

Genetic diversity of cyanobacterial communities in Lake Kinneret (Israel) using 16S rRNA gene, psbA and ntcA sequence analyses

Pilar Junier1,2,*, Karl-Paul Witzel2, Ora Hadas3

1École Polytechnique Fédérale de Lausanne, 1015 Lausanne, Switzerland
2Max Planck Institute for Limnology, August-Thienemann-Str. 2, 24306 Plön, Germany
3Israel Oceanographic and Limnological Research, the Kinneret Limnological Laboratory, PO Box 447, 14950 Migdal, Israel

ABSTRACT: The genetic diversity of cyanobacterial communities was studied at various depths in Lake Kinneret (Israel). Denaturing gradient gel electrophoresis (DGGE) of specific 16S rRNA gene PCR products showed significant differences in the cyanobacterial community structure between epi- and hypolimnetic waters. Sequences of clone libraries prepared from 16S rRNA gene PCR products from epi- and hypolimnion revealed the presence of at least 11 different groups of cyanobacteria. Clones related to the unicellular cyanobacteria (Chroococcales and picocyanobacteria) dominated the clone libraries from both depths. New primers to amplify the gene coding for the photosystem II reaction centre ( psbA) and the nitrogen regulator gene (ntcA) of cyanobacteria were developed and used for further characterization of the cyanobacterial communities from the lake. Sequences of psbA amplicons clustered with those from 2 different groups of marine Synechococcus and Chroococcales. Cloned ntcA amplicons from the lake were closely related and did not cluster with sequences from cultured cyanobacteria or other environmental sequences from this gene. All the molecular markers analyzed here showed similarity to sequences from some groups of cyanobacteria in the lake and those so far found in marine habitats.

KEY WORDS: Gloeocapsa · Picophytoplankton · 16S rRNA gene · psbA · ntcA

Full text in pdf format 
Cite this article as: Junier P, Witzel K, Hadas O (2007) Genetic diversity of cyanobacterial communities in Lake Kinneret (Israel) using 16S rRNA gene, psbA and ntcA sequence analyses. Aquat Microb Ecol 49:233-241.

Export citation
Mail this link - Contents Mailing Lists - RSS
Facebook - - linkedIn