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Aquatic Microbial Ecology

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AME 55:203-207 (2009)  -  DOI:

Protein modifications related to phage resistance in a marine roseobacter

Yongyu Zhang1,2, Nianzhi Jiao2,*, David R. Colquhoun3, Rolf U. Halden3,4, Feng Chen1,**

1Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, Maryland 21202, USA
2State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, 361005, PR China
3Department of Environmental Health Sciences, Center for Water and Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland 21205–2179, USA
4Center for Environmental Biotechnology, The Biodesign Institute at Arizona State University, Tempe, Arizona 85287–5701, USA

ABSTRACT: The Roseobacter lineage is an important group of bacteria in the marine environment. Silicibacter pomeroyi DSS-3 has been used as a model strain for understanding the biological and ecological relevance of this group. Recently, a phage (Φ1) which infects S. pomeroyi DSS-3 was isolated in our laboratory. Mutants of DSS-3 that are resistant to phage Φ1 were also obtained. A comparison of the protein expression profiles of the wild type, DSS-3, and the phage Φ1-resistant mutant M1 identified a potential phage resistance response due to the modification of 4 highly expressed proteins. One of these 4 proteins was successfully identified via mass spectrometry, and matched a hypothetical protein (SPOA0343) in the S. pomeroyi DSS-3 genome. Although the type of protein modification has not yet been determined, our results suggest that bacteria could become resistant to phage infection via protein modification. Studies on phage resistance of S. pomeroyi DSS-3 could provide new insight into the complex relationship between marine bacteria and their viruses in the natural environment.

KEY WORDS: Silicibacter pomeroyi DSS-3 · Phage resistance · Two-dimensional polyacrylamide gel electrophoresis · Mass spectrometry · Protein modification

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Cite this article as: Zhang Y, Jiao N, Colquhoun DR, Halden RU, Chen F (2009) Protein modifications related to phage resistance in a marine roseobacter. Aquat Microb Ecol 55:203-207.

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