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Aquatic Microbial Ecology

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AME 74:107-119 (2015)  -  DOI:

Diversity of quorum sensing autoinducer synthases in the Global Ocean Sampling metagenomic database

Margot Doberva1, Sophie Sanchez-Ferandin2, Eve Toulza3,4, Philippe Lebaron1, Raphaël Lami1,*

1Sorbonne Universités, UPMC Univ Paris 06, CNRS, Laboratoire de Biodiversité et Biotechnologie Marines (LBBM), Observatoire Océanologique, 66650 Banyuls/Mer, France
2Sorbonne Universités, UPMC Univ Paris 06, CNRS, Biologie Intégrative des Organismes Marins (BIOM), Observatoire Océanologique, 66650, Banyuls/Mer, France
3UPVD Université de Perpignan, UMR 5244, IHPE, 66860 Perpignan cedex 9, France
4CNRS, UMR 5244, 2EI, 66860 Perpignan cedex 9, France
*Corresponding author:

ABSTRACT: Quorum sensing (QS) is a cell-to-cell signalling pathway that allows bacteria to synchronize their genetic expression. It is mediated by autoinducers (AI), including (1) acyl-homoserine lactones (AHLs or AI-1), produced by Proteobacteria using AinS, LuxI and HdtS synthase families and (2) furanosyl-diester-borate (FDB or AI-2), produced by a large range of phylogenetically diverse bacteria and synthetized by the LuxS family. Few data have been collected about the presence and importance of QS in marine waters using culture independent methods. In this study, we examined the presence and the diversity of AI-1 and AI-2 synthases in the Global Ocean Sampling (GOS), a large metagenomic database, covering 68 stations across 3 oceans. We built 4 reference protein databases with maximal phylogenetic coverage containing all known AI synthase sequences to retrieve AI synthases sequences from the GOS metagenomes. We retrieved 29 environmental sequences affiliated to LuxI (synthesizing AI-1), 653 related to HdtS (AI-1), 31 related to LuxS (AI-2) and only one for AinS (AI-1). AI synthases sequences were found in the 3 oceans covered by the GOS cruise and spanned a large phylogenetic diversity. These data revealed a large number of new marine AI sequences, suggesting that QS based on AI-1 diffusion is a widespread mechanism in the marine environment.

KEY WORDS: Quorum sensing · Acyl-homoserine lactone synthases · Global Ocean Sampling · Environmental metagenomes

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Cite this article as: Doberva M, Sanchez-Ferandin S, Toulza E, Lebaron P, Lami R (2015) Diversity of quorum sensing autoinducer synthases in the Global Ocean Sampling metagenomic database. Aquat Microb Ecol 74:107-119.

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