ABSTRACT: In Bahía de la Ascensión in Mexico, the Caribbean spiny lobster Panulirus argus fishery is based on extensive use of artificial shelters (casitas) that can harbor both juveniles and adults of this highly gregarious species. There is concern that the use of casitas might increase contact transmission of Panulirus argus virus 1 (PaV1). However, a previous study found no evidence for lobster crowding within casitas influencing the prevalence of clinical PaV1 disease, although differences in clinical prevalence were noted between different bay environments. To investigate this more closely, 683 lobsters were sampled from casitas in 2 zones in this bay (Vigía Chico, a shallow low-vegetation zone, and Punta Allen, a deeper dense-vegetation zone) previously found to have the lowest and highest prevalence, respectively, of observed clinical signs. When hemolymph collected from these lobsters was tested by PCR, the prevalence of PaV1 infection was found to be significantly lower in Vigía Chico relative to Punta Allen irrespective of season or the size, sex, or presence of shell injuries on lobsters. Among 714 large commercial-catch lobsters collected throughout the bay, the prevalence of infection was low irrespective of year or sex. For all lobsters tested, the sensitivity (0.510) at which PaV1 infection was detected by observed clinical signs was about half that determined by PCR, but the specificity of clinical signs was absolute (1), indicating that a simple 2× correction factor can be used to accurately estimate PaV1 infection prevalence based on more easily conducted visual assessments of lobsters.