DAO 109:107-115 (2014)  -  DOI: https://doi.org/10.3354/dao02730

Full genome sequence of a novel circo-like virus detected in an adult European eel Anguilla anguilla showing signs of cauliflower disease

Andor Doszpoly1,*, Zoltán L. Tarján1, Róbert Glávits2, Tamás Müller3, Mária Benkő

1Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, PO Box 18, 1581 Budapest, Hungary
2Veterinary Diagnostic Directorate, National Food Chain Safety Office, Hungaria krt. 21, 1143 Budapest, Hungary
3Department of Fish Culture, Szent István University, 2100 Gödöllő, Hungary
*Corresponding author:

ABSTRACT: An adult European eel Anguilla anguilla, showing typical signs of the so-called cauliflower disease, was subjected to pathological and molecular virological examinations. Samples taken from internal organs and the polypoid proliferative tissue from the mouth were examined by PCR for the detection of several viruses. Positive results were obtained with a nested PCR targeting the rep gene of circoviruses. Analysis of the partial rep sequence indicated the presence of a putative novel circovirus, but attempts to isolate it remained unsuccessful. The missing part of the genome was acquired by an inverse nested PCR with 2 specific primer pairs, designed from the newly determined rep sequence, followed by genome walking. The circular full genome was found to consist of 1378 nt (GenBank accession no. KC469701). Two oppositely oriented open reading frames (ORFs) were present, of which one was unambiguously identified as a circoviral rep gene. However, the predicted product of the other ORF, though it is a clear positional counterpart of the cap genes, showed no obvious homology to any known circoviral capsid proteins. A stem-loop-like element in the intergenic region between the 5’ ends of the ORFs was also found. Phylogenetic calculations indicated that the novel virus belongs to the genus Circovirus in the family Circoviridae. The relative amount of the viral DNA in the organ samples was estimated by quantitative real-time PCR. The results suggested that the examined fish was caught in an active viremic state, although the role of this circovirus in the etiology of the cauliflower diseases could not be ascertained.

KEY WORDS: Cauliflower disease · Stomatopapilloma · Fish circovirus · Complete genome · Phylogeny

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Cite this article as: Doszpoly A, Tarján ZL, Glávits R, Müller T, Benkő M (2014) Full genome sequence of a novel circo-like virus detected in an adult European eel Anguilla anguilla showing signs of cauliflower disease. Dis Aquat Org 109:107-115. https://doi.org/10.3354/dao02730

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