DAO 109:117-126 (2014)  -  DOI: https://doi.org/10.3354/dao02725

Yersinia ruckeri Biotypes 1 and 2 in France: presence and antibiotic susceptibility

Ségolène Calvez1,2,*, Hubert Gantelet3, Guillaume Blanc1, Diane-Gaëlle Douet4, Patrick Daniel5

1LUNAM Université, Oniris, Ecole nationale vétérinaire, agroalimentaire et de l’alimentation Nantes-Atlantique, UMR 1300 Biologie, Epidémiologie et Analyse de Risque en santé animale, La Chantrerie, Nantes 44307 Cedex 3, France
2INRA, UMR1300, 44307 Nantes, France
3Biovac, 49071 Beaucouzé, France
4GDSAA, Groupe de Défense Sanitaire Aquacole Aquitain, 40000 Mont de Marsan, France
5LD40, Laboratoire d’analyse, 40000 Mont de Marsan, France
*Corresponding author:

ABSTRACT: Yersinia ruckeri is the causative agent of yersiniosis, a disease reported in a number of fish species, especially rainbow trout. This study was undertaken to describe the phenotypes of Y. ruckeri on French rainbow trout farms. More than 100 isolates, collected during recent outbreaks on trout farms, were characterized by phenotypic tests, namely using biochemical tests of the API 20E system, serotyping, biotyping (tests for motility and lipase activity) and by describing the pattern of susceptibility to several antibiotics. The isolates showed a low phenotypic diversity with a prevalent serotype (O1) and API 20E profile 5 1(3)07 100. As in other European countries, Biotype 2 (BT2), which lacks both motility and secreted lipase activity, was found to be present in France. The emergence of ‘French’ BT2 was different than that observed for other European countries (Finland, Spain, Denmark and the UK). The antibiotic pattern was uniform for all isolates, regardless of the geographical area studied. The results indicate that no resistance has yet emerged, and the efficacy of the antibiotic generally used against yersiniosis in France, trimethoprim/sulfamethoxasol, is not compromised (minimum inhibitory concentrations [MIC] of between 0.016 and 0.128 µg ml-1). Enrofloxacin and doxycycline, not used as a first-line treatment in fish diseases, have reasonably good efficacies (with MICs ≤0.128 and 0.256, respectively).

KEY WORDS: Yersiniosis · Serotype · Motility · Lipase activity · Resistance · Antimicrobial

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Cite this article as: Calvez S, Gantelet H, Blanc G, Douet DG, Daniel P (2014) Yersinia ruckeri Biotypes 1 and 2 in France: presence and antibiotic susceptibility. Dis Aquat Org 109:117-126. https://doi.org/10.3354/dao02725

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