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Diseases of Aquatic Organisms

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DAO 109:187-199 (2014)  -  DOI:

C-reactive protein and complement as acute phase reactants in common carp Cyprinus carpio during CyHV-3 infection

Nicolas Pionnier1, Mikołaj Adamek2, Joanna J. Miest1, Sarah J. Harris1,2, Marek Matras3, Krzysztof Ł. Rakus4,5, Ilgiz Irnazarow4, Dave Hoole1,*

1Institute of Science and Technology in Medicine, School of Life Sciences, Keele University, ST5 5BG Keele, UK
2Fish Diseases Research Unit, Centre for Infection Medicine, University of Veterinary Medicine in Hanover, Bünteweg 17, 30559 Hanover, Germany
3Laboratory of Fish Diseases, National Veterinary Research Institute, Partyzantów 57, 24-100 Puławy, Poland
4Polish Academy of Sciences, Institute of Ichthyobiology & Aquaculture in Gołysz, Kalinowa 2, 43-520 Chybie, Poland
5Present address: Immunology-Vaccinology (B43b), Department of Infectious and Parasitic Diseases (B43b), Faculty of Veterinary Medicine, University of Liège, 4000 Liège, Belgium
*Corresponding author:

ABSTRACT: Cyprinid herpesvirus 3 (CyHV-3) is the aetiological agent of a highly virulent and lethal disease of common carp Cyprinus carpio and its ornamental koi varieties. However, specific knowledge about immune mechanisms behind the infection process is very limited. We aimed to evaluate the effect of the CyHV-3 infection on the profile of 2 major components of the common carp immune acute phase response: the C-reactive protein (CRP) and the complement system. Common carp were infected with CyHV-3 by bath immersion. Fish were sampled before the infection and at 6, 12, 24, 72, 120 and 336 h post-infection for serum and head kidney, liver, gill and spleen tissues. CRP levels and complement activity were determined from the serum, whereas CRP- and complement-related genes (crp1, crp2, c1rs, bf/c2, c3, masp2) expression profiles were analysed in the tissues by quantitative PCR. Both CRP levels and complement activity increased significantly up to 10- and 3-fold, respectively, in the serum of infected fish during the challenge. Analysis revealed distinct organ- and time-dependent expression profile patterns for all selected genes. These results suggest that CRP and complement behave as acute phase reactants to CyHV-3 infection in common carp with an organ- and time-dependent response.

KEY WORDS: CRP · Complement · Cyprinid herpesvirus 3 · KHV · Koi herpesvirus · Carp · Biomarker

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Cite this article as: Pionnier N, Adamek M, Miest JJ, Harris SJ and others (2014) C-reactive protein and complement as acute phase reactants in common carp Cyprinus carpio during CyHV-3 infection. Dis Aquat Org 109:187-199.

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