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Diseases of Aquatic Organisms

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DAO 112:131-138 (2014)  -  DOI:

Isolation and characterization of a novel reovirus from white bream Blicca bjoerkna

Oskar Schachner1, Hatem Soliman1,2, Michael Straif3, Franz Schilcher4, Mansour El-Matbouli1,*

1Clinical Division of Fish Medicine, University of Veterinary Medicine, Veterinaerplatz 1, 1210 Vienna, Austria
2Fish Medicine and Managements, Faculty of Veterinary Medicine, Assiut University, 71515 Assiut, Egypt
3Institute of Hydrobiology and Aquatic Ecosystem Management, Department of Water - Atmosphere - Environment, University of Natural Resources and Applied Life Sciences, 1180 Vienna, Austria
4Institute of Pathology and Forensic Veterinary Medicine, Department of Pathobiology, University of Veterinary Medicine, 1210 Vienna, Austria
*Corresponding author:

ABSTRACT: During a fish health inspection in the Viennese waterway ‘Old Danube’, a virus was isolated exclusively from white bream Blicca bjoerkna (L.) (formerly Abramis bjoerkna L.), one of the most abundant cyprinids present and not known as a host species for this virus. The virus preferentially replicated in cultures of the epithelioma papulosum cyprini cell line where focal plaques of infection developed slowly. Examination of infected cell cultures by electron microscopy revealed non-enveloped 60 to 70 nm icosahedral virions that had characteristic multiple segregated protrusions of their outer capsid. A partial RNA-dependent RNA polymerase gene sequence was obtained and a BLAST search indicated 76% identity to golden shiner reovirus and grass carp reovirus. These results suggested that the virus belonged to the genus Aquareovirus (Family Reoviridae). Phylogenetic analysis placed the isolated virus within a clade of the species Aquareovirus C species. Accordingly, the virus was tentatively designated as white bream reovirus (WBRV) strain A-127/06 within the species Aquareovirus C.

KEY WORDS: Aquareovirus · White bream · Blicca bjoerkna · Cell line susceptibility · Gill histopathology · Electron microscopy · Phylogenetic analysis

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Cite this article as: Schachner O, Soliman H, Straif M, Schilcher F, El-Matbouli M (2014) Isolation and characterization of a novel reovirus from white bream Blicca bjoerkna. Dis Aquat Org 112:131-138.

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