DAO 113:51-58 (2015)  -  DOI: https://doi.org/10.3354/dao02816

Perkinsus sp. infections and in vitro isolates from Anadara trapezia (mud arks) of Queensland, Australia

Cécile Dang1,4,*, Christopher F. Dungan2, Gail P. Scott3, Kimberly S. Reece

1The University of Queensland, School of Biological Sciences and Centre for Marine Science, Brisbane, QLD 4072, Australia
2Maryland Department of Natural Resources, Cooperative Oxford Laboratory, 904 S. Morris Street, Oxford, Maryland 21654, USA
3Virginia Institute of Marine Science, College of William & Mary, PO Box 1346, Gloucester Point, Virginia 23062, USA
4Present address: Department of Fisheries, Government of Western Australia, Perth, WA 6151, Australia
*Corresponding author:

ABSTRACT: Perkinsus sp. protists were found infecting Anadara trapezia mud ark cockles at 6 sites in Moreton Bay, Queensland, Australia, at prevalences of 4 to 100% during 2011 as determined by surveys using Ray’s fluid thioglycollate medium. Perkinsus sp. lesions were found among gill and visceral connective tissues in histological samples from several cockles, where basophilic, eccentrically vacuolated Perkinsus sp. signet ring trophozoites and proliferating, Perkinsus sp. schizont cells were documented. Two Perkinsus sp. isolates were propagated in vitro during August 2013 from gill tissues of a single infected A. trapezia cockle from Wynnum in Moreton Bay. DNA from those isolate cells amplified universally by a Perkinsus genus-specific PCR assay, and rDNA-internal transcribed spacer sequences respectively grouped them with P. olseni and P. chesapeaki in phylogenetic analyses. This is the first report of P. chesapeaki in Australia, and the first report of a P. chesapeaki in vitro isolate from an Australian mollusc host. Although P. olseni was originally described in 1981 as a pathogen of abalone in South Australia, and has subsequently been identified as a prevalent pathogen of numerous other molluscs worldwide, this is also the first report of a P. olseni-like in vitro isolate from an Australian mollusc host.

KEY WORDS: Cockle · Perkinsus olseni · Perkinsus chesapeaki · RFTM

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Cite this article as: Dang C, Dungan CF, Scott GP, Reece KS (2015) Perkinsus sp. infections and in vitro isolates from Anadara trapezia (mud arks) of Queensland, Australia. Dis Aquat Org 113:51-58. https://doi.org/10.3354/dao02816

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