DAO 113:9-23 (2015)  -  DOI: https://doi.org/10.3354/dao02820

Cell-surface properties of Vibrio ordalii strains isolated from Atlantic salmon Salmo salar in Chilean farms

P. Ruiz1,2, M. Poblete1,2, A. J. Yáñez3,2, R. Irgang1,2, A. E. Toranzo4, R. Avendaño-Herrera1,2,*

1Laboratorio de Patología de Organismos Acuáticos y Biotecnología Acuícola, Facultad de Ciencias Biológicas, Universidad Andrés Bello, Viña del Mar, Chile
2Interdisciplinary Center for Aquaculture Research (INCAR), O’Higgins 1695, Concepción, Chile
3Instituto de Bioquímica y Microbiología, Facultad de Ciencias, Universidad Austral de Chile, Valdivia, Chile
4Departamento de Microbiología y Parasitología, CIBUS-Facultad de Biología. Universidad de Santiago de Compostela, Santiago de Compostela, Spain
*Corresponding author: ,

ABSTRACT: Vibrio ordalii is the causative agent of atypical vibriosis and has the potential to cause severe losses in salmonid aquaculture, but the factors determining its virulence have not yet been elucidated. In this work, cell-surface-related properties of the isolates responsible for outbreaks in Atlantic salmon were investigated. We also briefly examined whether pathogenicity against fish varied for V. ordalii strains with differing cell-surface properties. Hydrocarbon adhesions indicated the hydrophobic character of V. ordalii, although only 4 of 18 isolates induced haemagglutination in Atlantic salmon erythrocytes. A minority of the studied isolates (6 of 18) and the type strain ATCC 33509T produced low-grade biofilm formation on polyethylene surface after 2 h post-inoculation (hpi), but no strains were slime producers. Interestingly, V. ordalii isolates showed wide differences in hydrophobicity. Therefore, we chose 3 V. ordalii isolates (Vo-LM-03, Vo-LM-18 and Vo-LM-16) as representative of each hydrophobicity group (strongly hydrophobic, relatively hydrophobic and quasi-hydrophilic, respectively) and ATCC 33509T was used in the pathogenicity studies. All tested V. ordalii strains except the type strain resisted the killing activity of Atlantic salmon mucus and serum, and could proliferate in these components. Moreover, all V. ordalii isolates adhered to SHK-1 cells, causing damage to fish cell membrane permeability after 16 hpi. Virulence testing using rainbow trout revealed that isolate Vo-LM-18 was more virulent than isolates Vo-LM-03 and Vo-LM-16, indicating some relationship between haemagglutination and virulence, but not with hydrophobicity.

KEY WORDS: Vibrio ordalii · Cell-surface properties · Vibriosis · Atlantic salmon · Chile

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Cite this article as: Ruiz P, Poblete M, Yáñez AJ, Irgang R, Toranzo AE, Avendaño-Herrera R (2015) Cell-surface properties of Vibrio ordalii strains isolated from Atlantic salmon Salmo salar in Chilean farms. Dis Aquat Org 113:9-23. https://doi.org/10.3354/dao02820

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