DAO 113:227-234 (2015)  -  DOI: https://doi.org/10.3354/dao02850

ABSTRACT: The use of chemical pesticides has increased environmental pollution and affects fishes as non-target organisms. To investigate the toxic effects of the widely used herbicide atrazine on Caspian kutum Rutilus frisii kutum fingerlings, fish were exposed to a sublethal concentration of half LC₅₀ for 96 h. The main alterations visible in the gill tissue were detachment of the epithelium of the lamellae, necrosis, lamellar fusion, hyperplasia, club shaped lamellae, collapse of the lamellae, shrinkage and curling of the lamellae, and ultrastructural alterations such as necrosis of the apical microridges of the pavement cells. Results also showed that the gill ionocytes were fewer in number and larger in size in the atrazine-exposed fish. Atrazine appears to be highly toxic to Caspian kutum fingerlings even at a sublethal concentration (12.47 mg l^-1) and acute exposure. This toxicity could affect gill respiration and ion regulation function of fingerlings by damaging tissue, pavement cells, and ionocytes.

KEY WORDS: Ionocyte · Osmoregulation · Toxicology · Immunohistochemistry · Ultrastructure