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Diseases of Aquatic Organisms

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DAO 114:11-20 (2015)  -  DOI:

Genotyping WSSV isolates from northwestern Mexican shrimp farms affected by white spot disease outbreaks in 2010-2012

Ma. de Jesús Durán-Avelar1, Ricardo Pérez-Enríquez2, José Francisco Zambrano-Zaragoza1, Leobardo Montoya-Rodríguez3, Ricardo Vázquez-Juárez2, Norberto Vibanco-Pérez1,*

1Universidad Autónoma de Nayarit, Unidad Académica de Ciencias Químico Biológicas y Farmacéuticas, Tepic 63000, Nayarit, Mexico
2Centro de Investigaciones Biológicas del Noroeste S.C., La Paz 23096, Baja California Sur, Mexico
3Centro de Investigación en Alimentación y Desarrollo, Mazatlán 82000, Sinaloa, Mexico
*Corresponding author: 

ABSTRACT: White spot disease (WSD) causes high mortality in cultured shrimp throughout the world. Its etiologic agent is the white spot syndrome virus (WSSV). The genomic repeat regions ORF 75, ORF 94, and ORF 125 have been used to classify WSSV isolates in epidemiological studies using PCR with specific primers and sequencing. The present study investigated the variation in nucleotide sequences from 107, 150, and 143 isolates of WSSV collected from Litopenaeus vannamei shrimp ponds with WSD outbreaks in northwestern Mexico during the period 2010-2012, in the genomic repeat regions ORFs 75, 94, and 125, respectively. The haplotypic nomenclature for each isolate was based on the number of repeat units and the position of single nucleotide polymorphisms on each ORF. We report finding 17, 43, and 66 haplotypes of ORFs 75, 94, and 125, respectively. The study found high haplotypic diversity in WSSV using the complete sequences of ORFs 94 and 125 as independent variables, but low haplotypic diversity for ORF 75. Different haplotypes of WSSV were found from region-to-region and year-to-year, though some individual haplotypes were found in different places and in more than one growing cycle. While these results suggest a high rate of mutation of the viral genome at these loci, or perhaps the introduction of new viral strains into the area, they are useful as a tool for epidemiological surveys. Two haplotypes from some of the ORFs in the same shrimp were encountered, suggesting the possibility of multiple infections.

KEY WORDS: White spot syndrome virus · ORF 75 · ORF 94 · ORF 125 · Haplotypes · Litopenaeus vannamei · Whiteleg shrimp · Northwestern Mexico · Epidemiological studies

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Cite this article as: Durán-Avelar MdJ, Pérez-Enríquez R, Zambrano-Zaragoza JF, Montoya-Rodríguez L, Vázquez-Juárez R, Vibanco-Pérez N (2015) Genotyping WSSV isolates from northwestern Mexican shrimp farms affected by white spot disease outbreaks in 2010-2012. Dis Aquat Org 114:11-20.

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