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Diseases of Aquatic Organisms

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DAO 116:251-254 (2015)  -  DOI: https://doi.org/10.3354/dao02919

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Acute hepatopancreatic necrosis disease (AHPND) outbreaks in Penaeus vannamei and P. monodon cultured in the Philippines

Leobert D. de la Peña1,*, Nikko Alvin R. Cabillon1, Demy D. Catedral1, Edgar C. Amar1, Roselyn C. Usero2, Wilberto D. Monotilla3, Adelaida T. Calpe4, Dalisay DG. Fernandez4, Cynthia P. Saloma3

1Aquaculture Department, Southeast Asian Fisheries Development Center (SEAFDEC/AQD), Tigbauan, Iloilo 5021, Philippines
2Negros Prawn Producers Cooperative (NPPC), Bacolod City, Negros Occidental 6100, Philippines
3Philippine Genome Center (PGC), University of the Philippines Diliman, Quezon City 1101, Philippines
4Philippine Council for Agriculture, Aquatic and Natural Resources Research and Development (PCAARRD), Paseo de Valmayor, Timugan Los Baños, Laguna 4030, Philippines
*Corresponding author:

ABSTRACT: Acute hepatopancreatic necrosis disease (AHPND) has recently emerged as a serious disease of cultured shrimp. It has also been described as early mortality syndrome (EMS) due to mass mortalities occurring within 20 to 30 d after stocking of ponds with postlarvae. Here, Penaeus vannamei and Penaeus monodon from shrimp farms in the Philippines were examined for the toxin-producing strain of Vibrio parahaemolyticus due to AHPND-like symptoms occurring in marketable size shrimp. In the P. vannamei, histology revealed typical AHPND pathology, such as sloughing of undifferentiated cells in the hepatopancreatic tubule epithelium. Analysis using the IQ2000 AHPND/EMS Toxin 1 PCR test generated 218 bp and 432 bp amplicons confirmative of the toxin-producing strain of V. parahaemolyticus among shrimp sampled from 8 of 9 ponds. In the P. monodon, histology revealed massive sloughing of undifferentiated cells of the hepatopancreatic tubule epithelium in the absence of basophilic bacterial cells. PCR testing generated the 2 amplicons confirmatory for AHPND among shrimp sampled from 5 of 7 ponds. This study confirms the presence of AHPND in P. vannamei and P. monodon farmed in the Philippines and suggests that the disease can also impact late-stage juvenile shrimp.


KEY WORDS: AHPND · P. vannamei · P. monodon · PCR · Histopathology · Philippines


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Cite this article as: de la Peña LD, Cabillon NAR, Catedral DD, Amar EC and others (2015) Acute hepatopancreatic necrosis disease (AHPND) outbreaks in Penaeus vannamei and P. monodon cultured in the Philippines. Dis Aquat Org 116:251-254. https://doi.org/10.3354/dao02919

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