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Diseases of Aquatic Organisms

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DAO 117:229-235 (2016)  -  DOI:

Lacaziosis-like disease in Tursiops truncatus from Brazil: a histopathological and immunohistochemical approach

Carlos Sacristán1,*, Rodrigo Albergaria Réssio2, Pedro Castilho3, Natália Coelho Couto De Azevedo Fernandes2, Samira Costa-Silva1, Fernando Esperón4, Fábio Gonçalves Daura-Jorge5, Kátia R. Groch1, Cristiane K. M. Kolesnikovas6, Juliana Marigo1, Paulo Henrique Ott7,8, Larissa Rosa De Oliveira7,9, Angélica María Sánchez-Sarmiento1, Paulo C. Simões-Lopes5, José Luiz Catão-Dias1

1Departamento de Patologia, Laboratório de Patologia Comparada de Animais Selvagens, Faculdade de Medicina Veterinária  e Zootecnia, Universidade de São Paulo (LAPCOM-VPT-FMVZ-USP), Cidade Universitária, São Paulo, SP 05508 270, Brazil
2Instituto Adolfo Lutz, Cerqueira César, São Paulo, SP 01246-000, Brazil
3Departamento de Engenharia de Pesca, Universidade do Estado de Santa Catarina, Bairro Progresso, Laguna, SC 88.790-000, Brazil
4Grupo de Epidemiología y Sanidad Ambiental, Centro de Investigación en Sanidad Animal (INIA-CISA), Valdeolmos/Madrid 28130, Spain
5Departamento de Ecologia e Zoologia, Universidade Federal de Santa Catarina, Campus Universitário, s/n, Trindade, Florianópolis, SC 88040-970, Brazil
6Associação R3 Animal, Rodovia João Gualberto Soares (SC-406), Barra da Lagoa, Florianópolis, SC 88061-500, Brazil
7Grupo de Estudos de Mamíferos Aquáticos do Rio Grande do Sul - GEMARS, Osorio, RS 95595-000, Brazil
8Universidade Estadual do Rio Grande do Sul (UERGS), Bairro Sul brasileiro, Osório, RS 95595-000, Brazil
9Universidade do Vale do Rio dos Sinos - UNISINOS, São Leopoldo, RS 93022-000, Brazil
*Corresponding author:

ABSTRACT: Cetacean lacaziosis-like disease or lobomycosis-like disease (LLD) is a chronic skin condition caused by a non-cultivable yeast of the order Onygenales, which also includes Lacazia loboi, as well as Paracoccidioides brasiliensis and P. lutzii, respectively responsible for lacaziosis and paracoccidioidomycosis in humans. Complete identification and phylogenetic classification of the LLD etiological agent still needs to be elucidated, but preliminary phylogenetic analyses have shown a closer relationship of the LLD agent to Paracoccidioides spp. than to L. loboi. Cases of LLD in South American cetaceans based on photographic identification have been reported; however, to date, only 3 histologically confirmed cases of LLD have been described. We evaluated multiple tissue samples from 4 Tursiops truncatus stranded in the states of Santa Catarina (n = 3) and Rio Grande do Sul (n = 1), southern Brazil. Macroscopically, all animals presented lesions consistent with LLD. Hematoxylin-eosin, periodic acid-Schiff, Grocott’s methenamine silver, and Mayer’s mucicarmin stains were used for histological evaluation. Microscopically, numerous refractile yeasts (4-9 µm in diameter) were observed in skin samples (4/4), and for the first time in dolphins, also in a skeletal muscle abscess (1/4). Immunohistochemistry using anti-P. brasiliensis glycoprotein gp43 as a primary antibody, which is known to cross-react with L. loboi and the LLD agent, was performed and results were positive in all 4 cases. We describe 3 new cases of LLD in cetaceans based on histopathology and immunohistochemistry. This is the first report of LLD in the muscle of cetaceans.

KEY WORDS: Lobomycosis · Paracoccidioides brasiliensis · Cetacean · Yeast · Immunohistochemistry · Tursiops · Bottlenose dolphin

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Cite this article as: Sacristán C, Réssio RA, Castilho P, Fernandes NCCA and others (2016) Lacaziosis-like disease in Tursiops truncatus from Brazil: a histopathological and immunohistochemical approach. Dis Aquat Org 117:229-235.

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