DAO 118:217-226 (2016)  -  DOI: https://doi.org/10.3354/dao02976

Iron assimilation and siderophore production by Vibrio ordalii strains isolated from diseased Atlantic salmon Salmo salar in Chile

Pamela Ruiz1,2, Miguel Balado3, Alicia E. Toranzo3, Matías Poblete-Morales1,2, Manuel L. Lemos3,*, Ruben Avendaño-Herrera1,2,4,*

1Laboratorio de Patología de Organismos Acuáticos y Biotecnología Acuícola, Facultad de Ciencias Biológicas, Universidad Andrés Bello, Viña del Mar, Chile
2Interdisciplinary Center for Aquaculture Research (INCAR), Concepción, Chile
3Departamento de Microbiología y Parasitología. CIBUS-Facultad de Biología e Instituto de Acuicultura, Universidad de Santiago de Compostela, Santiago de Compostela 15782, Spain
4Centro de Investigación Marina Quintay (CIMARQ), Quintay, Chile
*Corresponding authors: ,

ABSTRACT: Vibrio ordalii is the causative agent of vibriosis in several cultured salmonid species worldwide. Despite its impact on aquaculture, relatively little information is available about its virulence factors. The present study demonstrates for the first time that V. ordalii possesses different systems of iron acquisition, one involving siderophore synthesis and another one that uses direct binding of heme to use iron. Using 6 strains of V. ordalii from Atlantic salmon Salmo salar and the V. ordalii type strain, we could demonstrate that all strains could grow in presence of the chelating agent 2,2’-dipyridyl and produced siderophores in solid and liquid media. Cross-feeding assays among V. ordalii strains evidenced variability in the siderophores produced. Bioassays and PCR data suggest that V. ordalii could produce a siderophore with a structure similar to piscibactin, although the production of a second siderophore in certain strains cannot be discarded. Furthermore, all strains were able to use hemin and hemoglobin as the only iron sources, although the cell yield was higher when using hemoglobin. A hemin-binding assay indicated the presence of constitutive heme-binding molecules at the cell surface of V. ordalii. Virulence tests using rainbow trout as a model of infection revealed a clear relationship between iron-uptake ability and pathogenicity in V. ordalii.

KEY WORDS: Vibrio ordalii · Siderophore · Iron sources · Virulence mechanisms · Atlantic salmon

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Cite this article as: Ruiz P, Balado M, Toranzo AE, Poblete-Morales M, Lemos ML, Avendaño-Herrera R (2016) Iron assimilation and siderophore production by Vibrio ordalii strains isolated from diseased Atlantic salmon Salmo salar in Chile. Dis Aquat Org 118:217-226. https://doi.org/10.3354/dao02976

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